| 货号 | AF4986-SP |
| 别名 | blood vessel epicardial substance; H; HBVES; POP1; POP1MGC42413; POPDC1; POPDC1popeye domain containing 1; Popeye domain-containing protein 1; Popeye protein 1 | 全称 | Blood Vessel Epicardial Substance |
| 反应种属 | Human |
| 应用 | Western Blot(1 µg/mL) |
| 目标/特异性 | Detects human BVES in direct ELISAs and Western blots. |
| 使用方法 | Western Blot: 1 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 11149 (Human); 23828 (Mouse) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human BVES Met1-Asn36 Accession # Q8NE79 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | BVES (blood vessel epicardial substance; also Popeye 1) is a 58 kDa, glycoprotein member of the Popeye family of multispan transmembrane (TM) molecules. It is expressed on embryonic epicardial epithelium plus atrial myocytes and on adult skeletal and cardiac muscle. It serves as a component of the epithelial tight junction, dimerizing with itself and interacting with ZO-1. Human BVES is 360 amino acids (aa) in length. It contains an extracellular N-terminus of 48 aa, three TM domains (aa 49‑113) and a large C-terminal cytoplasmic region (aa 114‑360) that contains a Popeye domain (aa 123‑266). Homodimerization occurs via aa 268‑274. There is one potential splice variant that shows a premature truncation after Glu213. Over aa 1‑36, human BVES is 72% aa identical to mouse BVES. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
Detection of Human BVES by Western Blot. Western blot shows lysates of human heart tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human BVES Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4986) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for BVES at approximately 58 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8. |
