| 货号 | AF4979-SP |
| 别名 | CIS2; CIS2CIS-2; Cish2; Cytokine-inducible SH2 protein 2; SOCS-2STAT-induced STAT inhibitor 2; SSI2; SSI-2STAT induced STAT inhibitor-2; SSI2STAT-induced STAT inhibitor-2; STATI2; STATI2suppressor of cytokine signaling-2; suppressor of cytokine signaling 2 | 全称 | Suppressor Of Cytokine Signaling 2 |
| 反应种属 | Human |
| 应用 | Western Blot(0.1 µg/mL) |
| 目标/特异性 | Detecs human SOCS-2 in Western blots. |
| 使用方法 | Western Blot: 0.1 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 8835 (Human); 84607 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human SOCS-2 Met1-Gln197 Accession # O14508 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | SOCS-2 (Suppressor of cytokine signaling 2; also CIS-2 and SSI-2) is a 25 kDa member of the SOCS family of proteins. It is an SH2-domain containing cytoplasmic adaptor protein expressed by multiple cell types. It blocks receptor signaling by binding to phosphorylated tyrosines in the cytoplasmic domain of select receptors, including the receptors for leptin, IGF-I and GH. Human SOCS-2 is 198 amino acids (aa) in length and contains one SH2 domain (aa 48‑156) and a SOCS box (aa 160‑198) that mediates interaction with elongin B or C of the E3 ubiquitin ligase complex. There is one splice variant that shows an Arg-rich eight aa substitution for aa 31‑39. Full-length SOCS-2 is 94% and 96% aa identical to mouse and porcine SOCS-2, respectively. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Human SOCS‑2 by Western Blot. Western blot shows lysates of K562 human chronic myelogenous leukemia cell line. Gels were loaded with 30 μg of whole cell lysate (WCL), 20 μg of cytoplasmic (Cyto), and 10 μg of nuclear extracts (Nuc). PVDF membrane was probed with 0.1 µg/mL Goat Anti-Human SOCS‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4979) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band for SOCS‑2 was detected at approximately 25 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. |
