| 货号 | AF4978-SP |
| 别名 | Zic family member 1 (odd-paired homolog, Drosophila); ZICZic family member 1 (odd-paired Drosophila homolog); Zinc finger protein 201; Zinc finger protein of the cerebellum 1; ZNF201; ZNF201zinc finger protein ZIC 1 | 全称 | Zic Family Member 1 |
| 反应种属 | Human/Mouse |
| 应用 | Western Blot(1 µg/mL) Immunohistochemistry(5-15 µg/mL) Immunocytochemistry(5-15 µg/mL) |
| 目标/特异性 | Detects human and mouse ZIC1 in direct ELISAs and Western blots. |
| 使用方法 | Western Blot: 1 µg/mL Immunohistochemistry: 5-15 µg/mL Immunocytochemistry: 5-15 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 7545 (Human); 22771 (Mouse); 64618 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human ZIC1 Met1-Gly198 Accession # Q15915 |
| 生物活性 | Human, Mouse |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | ZIC1 (zinc-finger protein of the cerebellum 1) is a 54-60 kDa member of the GLI C2H2-type Zn-finger protein family. It is expressed in embryonic sclerotome and dermomyotome and in postmitotic cerebellar granule cells and retinal ganglion cells. ZIC1 is a transcription factor that promotes ApoE expression and blocks activation of Math1, allowing for neuroblast multiplication at the expense of differentiation. Human ZIC1 is 447 amino acids (aa) in length. It contains N-terminal poly Ala and His segments, followed by five C2H2-type Zn-fingers. Although the first two are atypical (aa 225-296), the last three Zn-fingers (aa 302-384) are canonical and bind to GLI1, 2 and 3. There is one potential alternate start site at Met353. Over aa 1-198, human ZIC1 is 99% aa identical to mouse ZIC1. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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ZIC1 in Mouse Brain. ZIC1 was detected in immersion fixed frozen sections of mouse brain (cerebellum) using 15 µg/mL Goat Anti-Human/Mouse ZIC1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4978) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections. | |
Detection of Human/Mouse ZIC1 by Western Blot. Western blot shows lysates of human brain tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse ZIC1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4978) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for ZIC1 at approximately 54 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8. | |
ZIC1 in Human iPSK3 cells. ZIC1 was detected in immersion fixed human plasmid-derived induced pluripotent stem cells (iPSK3) using Goat Anti-Human/Mouse ZIC1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4978) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to nuclei and cytoplasm. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips. |
