| 货号 | AF4945-SP |
| 别名 | amyloid beta (A4) precursor-like protein 2; amyloid precursor protein homolog HSD-2; Amyloid protein homolog; amyloid-like protein 2; APLP-2; APPH; APPHCDEI box-binding protein; APPL2; CDEBP; WALPLP2; YWK-II | 全称 | Amyloid Beta [A4] Precursor-like Protein 2 |
| 反应种属 | Human |
| 应用 | Western Blot(1 µg/mL) |
| 目标/特异性 | Detects human APLP-2 in direct ELISAs and Western blots. In direct ELISAs, approximately 30% cross-reactivity with recombinant mouse (rm) APLP-2 is observed and less than 1% cross-reactivity with recombinant human APLP-1 and rmAPLP-1 is observed. |
| 使用方法 | Western Blot: 1 µg/mL |
| 来源 | Sterile PBS to a final concentration of 0.2 mg/mL. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 334 (Human); 11804 (Mouse); 64312 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | Mouse myeloma cell line NS0-derived recombinant human APLP-2 Leu37-Ser680 Accession # AAH00373 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Sterile PBS to a final concentration of 0.2 mg/mL. |
| 背景 | APLP-2 is a 100-170 kDa glycoprotein, member of the APP family of neuronal type I transmembrane proteins. Its extracellular domain consists of an N-terminal Cys-rich domain, an Asp/Glu-rich acidic region, a Kunitz protease inhibitor (KPI) domain, and a GAG attachment site in the membrane proximal domain. APLP-2 forms both homodimers and heterodimers with APP and APLP-1. Proteolytic cleavage of APLP-2 generates peptides similar to the amyloidogenic A beta peptides and a cytoplasmic fragment that functions as a transcriptional coactivator. Alternate splicing of APLP-2 generates isoforms that lack the KPI domain or contain an insertion that prevents GAG attachment. The extracellular domain of human APLP-2 shares 81% and 93% amino acid sequence identity with mouse and rat APLP-2, respectively. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Human APLP‑2 by Western Blot. Western blot shows lysates of SH‑SY5Y human neuroblastoma cell line. PVDF Membrane was probed with 1 µg/mL of Human APLP‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4945) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for APLP‑2 at approximately 115 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. |
