| 货号 | AF4936-SP |
| 别名 | FLJ43224; MGC104393; MGC125896; MGC125897; NEDD-8; Neddylin; Neural precursor cell expressed developmentally down-regulated protein 8; neural precursor cell expressed, developmentally down-regulated 8; Rub1; Ubiquitin-like protein Nedd8 | 全称 | Neural Precursor Cell Expressed Developmentally Down-Regulated Protein 8 |
| 反应种属 | Human/Mouse/Rat |
| 应用 | Western Blot(1 µg/mL) Immunohistochemistry(5-15 µg/mL) |
| 目标/特异性 | Detects human, mouse, and rat NEDD8 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 2% cross‑reactivity with recombinant human NEDD9 is observed. |
| 使用方法 | Western Blot: 1 µg/mL Immunohistochemistry: 5-15 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 4738 (Human); 18002 (Mouse); 25490 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human NEDD8 Leu2-Gly77 Accession # Q15843 |
| 生物活性 | Human, Mouse, Rat |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | NEDD8 (Neural precursor cell-expressed developmentally down-regulated gene 8; also Rub1) is a 6-8 kDa member of the ubiquitin family of proteins. It is expressed in striated muscle, and via its C-terminal Gly, forms covalent bonds with cullin family proteins. This conjugation generates ubiquitin ligase activity that regulates cell cycle proteins. Human pro-NEDD8 is 81 amino acids (aa) in length. It contains one ubiquitin-like domain (aa 1-65), and a 4-5 aa C-terminal propeptide that is cleaved to expose a C-terminal Gly residue that is used to generate a Gly-Lys intermolecular bond. Mature human NEDD8 shows 100% aa sequence identity to NEDD8 from both mouse and canine. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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NEDD8 in Human Skeletal Muscle. NEDD8 was detected in immersion fixed paraffin-embedded sections of human skeletal muscle using 15 µg/mL Sheep Anti-Human/Mouse/Rat NEDD8 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4936) overnight at 4 °C. Tissue was stained with the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections. | |
Detection of Human NEDD8 by Western Blot. Western blot shows lysates of K562 human chronic myelogenous leukemia cell line chronic myelogenous leukemia, Nalm‑6 human Pre‑B acute lymphocytic leukemia cell line, and HT1080 human fibrosarcoma cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human/Mouse/Rat NEDD8 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4936) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for NEDD8 at approximately 5 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8. |
