| 货号 | AF4935-SP |
| 别名 | FOSL1; FOS-like antigen 1; FOS-like antigen-1; fos-related antigen 1; FRA; FRA1; fra-1 | 全称 | Fos-related antigen 1 |
| 反应种属 | Human |
| 应用 | Western Blot(0.2 µg/mL) Immunohistochemistry(5-15 µg/mL) |
| 目标/特异性 | Detects human and mouse FRA-1 in Western blots. |
| 使用方法 | Western Blot: 0.2 µg/mL Immunohistochemistry: 5-15 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 8061 (Human); 14283 (Mouse); 25445 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human FRA-1 Gly146-Ala247 Accession # P15407 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | FRA-1 is a member of the leucine zipper Fos family of transcription factors. Fos family members have been associated with cellular proliferation, differentiation and transformation. In accordance with these findings, overexpression of FRA-1 correlates with a malignant phenotype in breast cancer. In lung epithelia cells, FRA-1 promotes motility, invasion, and anchorage independent growth. FRA-1 knock out mice rescued for the FRA-1 placental defect are viable but show reduced bone formation that may be due to a defect in the osteoblast differentiation. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Human FRA‑1 by Western Blot. Western blot shows lysates of A172 human glioblastoma cell line untreated (-) or treated (+) with 100 ng/mL Recombinant Human EGF (Catalog #236-EG) for 18 hours. PVDF membrane was probed with 0.2 µg/mL of Goat Anti-Human FRA‑1 Polyclonal Antibody (Catalog # AF4935), followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for FRA‑1 at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. | |
FRA‑1 in Human Melanoma. FRA‑1 was detected in immersion fixed paraffin-embedded sections of human melanoma using Goat Anti-Human FRA‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4935) at 3 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections. |
