| 货号 | AF4904-SP |
| 别名 | BRI3; BRI3E25C; BRICD2C; BRICHOS domain containing 2C; Cerebral protein 14; E25; hRPC.1050_D_4; integral membrane protein 2C; integral membrane protein 3; ITM3; Transmembrane protein BRI3 | 全称 | Integral Membrane Protein 2C |
| 反应种属 | Human |
| 应用 | Western Blot(1 µg/mL) |
| 目标/特异性 | Detects human ITM2C in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 1% cross-reactivity with recombinant human (rh) ITM2A and rhITM2B is observed. |
| 使用方法 | Western Blot: 1 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 81618 (Human); 64294 (Mouse); 301575 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human ITM2C Ala86-Val267 Accession # Q9NQX7 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | ITM2C (Integral membrane protein 2C; also BRI3 and cerebral protein 14) is a 36‑38 kDa member of the BRI3 family of proteins. It is found on neurons and appears to interact with APP, where ITM2C binding blocks proteolytic processing of APP, plus SCG10, where ITM2C binding blocks the microtubule-destabilizing activity of SCG10, thus inhibiting neurite outgrowth. Human ITM2C is a 267 amino acid (aa) type II transmembrane glycoprotein. Proteolytic cleavage by furin at Arg242Gly243 may create a truncated 34‑35 kDa ITM2C plus a C-terminal 4 kDa active peptide (aa 243‑267). The ECD (aa 76‑267) contains one BRICHOS domain (aa 136‑230). ITM2C may exist as a dimer. There are two splice variants, one that shows a deletion of aa 41‑87, and a second that shows a deletion of aa 151‑187. Over aa 86‑267, human ITM2C shares 96% aa identity with mouse ITM2C. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Human ITM2C by Western Blot. Western blot shows lysates of JEG‑3 human epithelial choriocarcinoma cell line and human brain tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human ITM2C Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4904) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for ITM2C at approximately 35‑36 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8. |
