| 货号 | AF4890-SP |
| 别名 | follistatin-like 4; Follistatin-like protein 4; follistatin-related protein 4; FSL4; FSTL4; KIAA1061 |
| 反应种属 | Human |
| 应用 | Western Blot(0.1 µg/mL) |
| 目标/特异性 | Detects human Follistatin-like 4/FSTL4 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 10% cross‑reactivity with recombinant mouse FSTL4 is observed and less than 1% cross-reactivity with recombinant human (rh) FSTL1 and rhFSTL5 is observed. |
| 使用方法 | Western Blot: 0.1 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 23105 (Human); 320027 (Mouse); 303130 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | Chinese hamster ovary cell line CHO-derived recombinant human Follistatin-like 4/FSTL4 Trp23-Val842 Accession # NP_055897 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | Follistatin-like 4 (FSTL4) is a presumably secreted member of the follistatin domain-containing protein family (1). Follistatin itself is a potent inhibitor of the TGF-beta superfamily member, activin (1, 2), and this activity is reflected in several other proteins of this family that contain varying numbers of the cysteine-rich follistatin domain. Many of these are matrix proteins such as SPARC and testican for which TGF-beta superfamily inhibition status is presumed but unknown (2, 3). R&D Systems recombinant human FSTL4 inhibits the ability of a TGF-beta superfamily member, BMP-6, to induce osteoblast differentiation of a mouse fibroblast cell line. Human FSTL4 cDNA encodes an 842 amino acid (aa) protein containing a 22 aa signal sequence, a follistatin (EGF- and kazal-like) domain, an EF-hand calcium-binding motif, and two Ig-like cell adhesion molecule-type (IgCAM) domains (1, 4). The second IgCAM domain contains a consensus sequence for FGF/FGF receptor interaction (4). Mature human FSTL4 shares 84%, 82% and 82% aa identity with mouse, rat and bovine FSTL4, respectively. Identity with other follistatin-like family members is mainly within consensus residues of the follistatin domain. Of two reported human FSTL4 isoforms, one is missing the follistatin and EF-hand domains, while the other contains all recognized domains, but is truncated after aa 600 (4). |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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