| 货号 | AF4888-SP |
| 别名 | CD301 antigen; CD301; CD301C-type lectin domain family 10 member A; CLECSF13; CLECSF14; CLECSF14macrophage C-type lectin; C-type (calcium dependent, carbohydrate-recognition domain) lectin, superfamilymember 14 (macrophage-derived); C-type lectin domain family 10, member A; C-type lectin superfamily member 14; HML2; HMLC-type (calcium dependent, carbohydrate-recognition domain) lectin, superfamilymember 13 (macrophage-derived); macrophage lectin 2 (calcium dependent); Macrophage lectin 2; MGL | 全称 | Macrophage Galactose N-Acetyl-Galactosamine Specific Lectin 1 |
| 反应种属 | Human/Mouse |
| 应用 | Western Blot(1 µg/mL) Flow Cytometry(2.5 µg/106cells) |
| 目标/特异性 | Detects human CLEC10A/CD301 in direct ELISAs and Western blots and mouse CLEC10A in Western blots. In this format, less than 1% cross-reactivity with recombinant human (rh) CLEC1, rhCLEC2, and rhCLEC3B is observed. |
| 使用方法 | Western Blot: 1 µg/mL Flow Cytometry: 2.5 µg/106cells |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 10462 (Human) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | Mouse myeloma cell line NS0-derived recombinant human CLEC10A/CD301 Gln61-His316 Accession # Q8IUN9 |
| 生物活性 | Human, Mouse |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | CLEC10A, also known as macrophage galactose/N-acetyl-galactosamine (GalNAc) specific lectin (MGL), CD301, DC-ASGPR, and HML, is a 35-40 kDa type II transmembrane glycoprotein that belongs to the C-type lectin family (1). Human and rat carry a single gene for CLEC10A/MGL, while mouse has two closely related MGL1 and MGL2 genes. Human CLEC10A/MGL consists of a 39 amino acid (aa) cytoplasmic region, a 21 aa transmembrane segment and a 256 aa extracellular domain (ECD) with one carbohydrate recognition domain (CRD) and a neck region (2). Within the CRD, human CLEC10A/MGL shares 64% - 70% aa sequence identity with mouse MGL1, mouse MGL2, and rat MGL. Alternate splicing generates multiple isoforms of human CLEC10A/MGL with 27 aa, 3 aa, and/or 4 aa deletions within the ECD (3, 4). CLEC10A/MGL is expressed on immature myleloid dendritic cells and alternatively activated (tolerogenic) macrophages and is upregulated by the immunosuppressant dexamethasone (3-7). CLEC10A/MGL selectively binds and internalizes terminal nonsialylated alpha - or beta -linked GalNAc moieties on O-linked carbohydrates, including the Tn carcinoma antigen (2-4, 8, 9). Similar ligand preference is exhibited by mouse MGL2 but not MGL1 (10). CLEC10A/MGL expressed on tolerogenic dendritic cells binds carbohydrate determinants on CD45 (RA, RB, and RC but not RO isoforms) expressed by T, NK, and B cells (6). This interaction inhibits effector T cell activation and induces their apoptosis (6). CLEC10A/MGL also binds the GP envelope glycoprotein on Marburg and Ebola viruses and enhances viral entry and infectivity (11). |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Human CLEC10A/CD301 by Western Blot. Western blot shows lysates of human immature dendritic cells and human mature dendritic cells. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse CLEC10A/CD301 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4888) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for CLEC10A/CD301 at approximately 35 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. | |
Detection of CLEC10A in Human Dendritic Cells by Flow Cytometry. Human immature dendritic cells were stained with Goat Anti-Human/Mouse CLEC10A/CD301 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4888, filled histogram) or isotype control antibody (Catalog # AB‑108‑C, open histogram), followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108). | |
Detection of Mouse CLEC10A/CD301 by Western Blot. Western blot shows lysates of mouse immature dendritic cells and mouse mature dendritic cells. PVDF membrane was probed with 2 µg/mL of Goat Anti-Human/Mouse CLEC10A/CD301 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4888) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for CLEC10A/CD301 at approximately 37 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. |
