| 货号 | AF4878-SP |
| 别名 | CALBL; DJ501N12.8; SCGN; SECRET; secretagogin, EF-hand calcium binding protein; SECRETsecretagogin; SEGN; Setagin |
| 反应种属 | Human/Mouse/Rat |
| 应用 | Western Blot(1 µg/mL) Simple Western(10 µg/mL) |
| 目标/特异性 | Detects mouse Secretagogin in Western blots. Reactivity with human, rat, and other species is expected due to the high sequence homology of the immunogen used. |
| 使用方法 | Western Blot: 1 µg/mL Simple Western: 10 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 10590 (Human); 214189 (Mouse); 306942 (Rat) |
| 应用文献 | |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Secretagogin is a Ca2+-binding protein identifying prospective extended amygdala neurons in the developing mammalian telencephalon. | |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human Secretagogin Asn164-Pro276 Accession # O76038 |
| 生物活性 | Human, Mouse, Rat |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | Secretagogin (SCGN) is a 32 kDa member of the EF-hand family of Ca-binding proteins. It is expressed in select neurons, as well as neuroendocrine cells and pancreatic islets. Human Secretagogin is 276 amino acids (aa) in length and contains six 35 aa EF-hand domains (aa 12‑276). There is one alternative splice variant that shows a 22 aa substitution for aa 28‑276, and which demonstrates no Ca-binding ability. Secretagogin is known to circulate, presumably released during cell apoptosis. Over aa 164‑276, human Secretagogin is more than 97% aa identical to mouse, porcine and canine Secretagogin. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
|
Detection of Human/Mouse/Rat Secretagogin by Western Blot. Western blot shows lysates of beta TC‑6 mouse beta cell insulinoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse/Rat Secretagogin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4878) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Secretagogin at approximately 32 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. | |
Detection of Human and Mouse Secretagogin by Simple WesternTM. Simple Western lane view shows lysates of beta TC‑6 mouse beta cell insulinoma cell line and human pancreas tissue, loaded at 0.2 mg/mL. A specific band was detected for Secretagogin at approximately 38 kDa (as indicated) using 10 µg/mL of Goat Anti-Human/Mouse/Rat Secretagogin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4878) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the12-230 kDa separation system. |
