| 货号 | AF4859-SP |
| 别名 | DDX58; DEAD (Asp-Glu-Ala-Asp) box polypeptide 58; DEAD box protein 58; DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide; DKFZp686N19181; EC 3.6.4.13; FLJ13599; probable ATP-dependent RNA helicase DDX58; retinoic acid inducible gene I; Retinoic acid-inducible gene 1 protein; Retinoic acid-inducible gene I protein; RIG-1; RIG-IDKFZp434J1111; RNA helicase RIG-I | 全称 | RIG-I |
| 反应种属 | Human |
| 应用 | Western Blot(1 µg/mL) |
| 目标/特异性 | Detects human RIG-I in direct ELISAs and Western blots. |
| 使用方法 | Western Blot: 1 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 23586 (Human); 230073 (Mouse) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human RIG-I Met724-Lys925 Accession # O95786 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | RIG-I (Retinoic-acid inducible gene I; also Dead-box protein 58) is a 100 kDa member of the DexH-box family of proteins. It is expressed in smooth muscle cells and endothelium. RIG-I is normally an inactive monomer. Upon Sendai and Hepatitis C virus infection, RIG-I binds to dsRNA, initiating homodimerization and downstream activation of IRF3 and KF kappa B genes. Human RIG-I is 925 amino acids (aa) in length. It contains two CARD domains (aa 1‑172), one helicase ATP-binding region (aa 251‑430), and one C-terminal repressor domain (aa 735‑925). There are three potential splice variants. One shows an alternate start site at Met204, while another shows deletion of aa 3‑80, and a third shows a deletion of aa 36‑80 plus 749‑925. Over aa 724‑925, human RIG-I is 76% aa identical to mouse RIG-I. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Human RIG-I by Western Blot. Western blot shows lysates of A172 human glioblastoma cell line and Nalm‑6 human Pre‑B acute lymphocytic leukemia cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human RIG-I Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4859) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for RIG-I at approximately 105 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8. |
