| 货号 | AF4857-SP |
| 别名 | 1810044J04Rik; B cell adaptor protein; BCAPB-cell adapter for phosphoinositide 3-kinase; B-cell phosphoinositide 3-kinase adapter protein 1; FLJ35564; phosphoinositide 3-kinase adapter protein 1; phosphoinositide-3-kinase adaptor protein 1; Pik3ap1; RP11-34E5.3 | 全称 | B Cell Adaptor Protein |
| 反应种属 | Human/Mouse |
| 应用 | Western Blot(0.5 µg/mL) |
| 目标/特异性 | Detects endogenous human and mouse BCAP in Western blots. |
| 使用方法 | Western Blot: 0.5 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 118788 (Human); 83490 (Mouse); 294048 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human BCAP Met462-Leu650 Accession # Q6ZUJ8 |
| 生物活性 | Human, Mouse |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | BCAP (B-cell adaptor for phosphoinositide 3-kinase (PI3K)) participates in linking the B cell antigen receptor (BCR) with the PI3K pathway. Tyrosine phosphorylation of BCAP by BCR-associated protein tyrosine kinases, such as Syk and Btk, generates binding sites for the p85 subunit of PI3K, resulting in activation of the PI3K pathway. Human and mouse BCAP contain 3 YXXM SH2 binding motifs for interaction with PI3K. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Human/Mouse BCAP by Western Blot. Western blot shows lysates of MO7e human megakaryocytic leukemic cell line and L1.2 mouse pro-B cell line. PVDF membrane was probed with 0.5 µg/mL of Human/Mouse BCAP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4857) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for BCAP at approximately 100 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 1. |
