| 货号 | AF4856-SP |
| 别名 | B47; Epidermal growth factor receptor GRB-7; GRB7 adapter protein; growth factor receptor-bound protein 7 | 全称 | Growth Factor Receptor-Bound Protein 7 |
| 反应种属 | Human/Mouse/Rat |
| 应用 | Western Blot(0.5 µg/mL) |
| 目标/特异性 | Detects endogenous human, mouse, and rat GRB7 in Western blots. |
| 使用方法 | Western Blot: 0.5 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 2886 (Human); 14786 (Mouse); 84427 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human GRB7 Met130-Tyr274 Accession # Q14451 |
| 生物活性 | Human, Mouse, Rat |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | GRB7 (Growth factor receptor-bound protein 7) mediates the coupling of multiple cell surface receptors to downstream signaling pathways in the regulation of various cellular functions, such as proliferation, apoptosis, and cell migration. GRB7 protein consists of several distinctive structural features, including an amino-terminal proline-rich region, a central GM region (for Grb and Mig), and a carboxyl-terminal SH2 domain. GRB7 is phosphorylated by focal adhesion kinase at cell adhesion sites. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
|
Detection of Human/Mouse/Rat GRB7 by Western Blot. Western blot shows lysates of CCD‑1070Sk human foreskin fibroblast cell line, NIH‑3T3 mouse embryonic fibroblast cell line and Rat‑2 rat embryonic fibroblast cell line. PVDF membrane was probed with 0.5 µg/mL of Human/Mouse/Rat GRB7 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4856) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for GRB7 at approximately 70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. |
