| 货号 | AF4847-SP |
| 别名 | Oncogene TCL1; Oncogene TCL-1; Protein p14 TCL1; T-cell leukemia/lymphoma 1A; T-cell lymphoma-1; T-cell lymphoma-1A; TCL1-PEN; TCL1T-cell leukemia/lymphoma protein 1A | 全称 | T Cell Leukemia Protein 1A |
| 反应种属 | Human |
| 应用 | Western Blot(1 µg/mL) Immunocytochemistry(5-15 µg/mL) |
| 目标/特异性 | Detects human TCL1A in Western blots. |
| 使用方法 | Western Blot: 1 µg/mL Immunocytochemistry: 5-15 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 8115 (Human); 21432 (Mouse); 690575 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human TCL1A Met1-Asp114 Accession # P56279 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | TCL1A (T cell leukemia protein 1A; also p14 TCL1) is a 14 kDa member of the TCL1 family of proteins. It is expressed by thymocytes and activated T and B lymphocytes, and interacts with Akt kinase, promoting its activation and the subsequent deactivation of proapoptotic molecules. Human TCL1A is 114 amino acids (aa) in length. The amino acids Asp16 and Ile74 mediate Akt association, while Pro36 Leu37 Thr38 mediates TCL1A homodimerization. Full length human TCL1A is 50% aa identical to mouse TCL1A. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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TCL1A in Daudi Human Burkitts Lymphoma Cells. TCL1A was detected in immersion fixed Daudi human Burkitts lymphoma cell line using 10 µg/mL Goat Anti-Human TCL1A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4847) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips. | |
Detection of Human TCL1A by Western Blot. Western blot shows lysates of Daudi human Burkitts lymphoma cell line and Ramos human Burkitts lymphoma cell line. PVDF membrane was probed with 1 µg/mL Goat Anti-Human TCL1A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4847) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). For additional reference, recombinant human TCL1A was included. A specific band for TCL1A was detected at approximately 14 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2. |
