| 货号 | AF4840-SP |
| 别名 | EndoPDI; ERp46; FLJ90810; HCC-2; member 15; MGC3178; PDIA15; thioredoxin domain containing 5 (endoplasmic reticulum); thioredoxin domain containing 5; thioredoxin domain-containing protein 5; thioredoxin related protein; TLP46 | 全称 | Thioredoxin Domain Containing 5 |
| 反应种属 | Human |
| 应用 | Western Blot(1 µg/mL) |
| 目标/特异性 | Detects endogenous human TXNDC5 in Western blots. |
| 使用方法 | Western Blot: 1 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 81567 (Human); 105245 (Mouse) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human TXNDC5 Glu110-Leu432 Accession # Q8NBS9 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | TXNDC5 (Thioredoxin (TRX) domain-contain protein 5; also ERp46 and EndoPDI) is a 50‑55 kDa member of the disulfide isomerase family of enzymes. It is expressed in hepatocytes, and tumor plus hypoxic endothelium. TXNDC5 demonstrates thioreductase (disulfide bond reduction) activity in the ER. Mature human TXNDC5 is 400 amino acids (aa) in length and migrates anomalously in SDS-PAGE. It contains three TRX domains (aa 36‑428) and a short ER retention motif (aa 429‑432). There are three potential splice variants. One shows an alternate start site at Met109, while two others show a 16 and 45 aa substitution for the N-terminal 88 amino acids. Over aa 110‑432, human TXNDC5 is 88% aa identical to mouse TXNDC5. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Human TXNDC5 by Western Blot. Western blot shows lysates of HT‑29 human colon adenocarcinoma cell line, A549 human lung carcinoma cell line, DU145 human prostate carcinoma cell line, and HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Human TXNDC5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4840) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for TXNDC5 at approximately 50-55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2. |
