| 货号 | AF4827-SP |
| 别名 | CD225 antigen; CD225; CD225Leu-13 antigen; IFI17; IFI17LEU13,9-27; interferon induced transmembrane protein 1 (9-27); Interferon-induced protein 17; interferon-induced transmembrane protein 1; Interferon-inducible protein 9-27; Leu-13 | 全称 | Interferon-induced Transmembrane Protein 1 |
| 反应种属 | Human |
| 应用 | Western Blot(1 µg/mL) Immunocytochemistry(5-15 µg/mL) |
| 目标/特异性 | Detects human IFITM1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 40% cross-reactivity with recombinant human (rh) IFITM2 and rhIFITM3 is observed. |
| 使用方法 | Western Blot: 1 µg/mL Immunocytochemistry: 5-15 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 8519 (Human); 293618 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human IFITM1 Met1-His36 Accession # NP_003632 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | IFITM1 (Interferon inducible transmembrane protein 1; also Leu13, CD225 and "9‑27") is a 17 kDa member of the CD225 family of proteins. It is inducible in multiple cell types by both type I and II interferons, and although reported to occur on the cell surface, is apparently concentrated in the ER. Homotypic IFITM1 interactions promote p53 antiproliferative activity by blocking p53 phosphorylation on Thr55. Human IFITM1 is a 2‑transmembrane protein 125 amino acids (aa) in length. The topology of the molecule is unclear, but transmembrane segments are reported between aa 37‑57 and 87‑107. Over aa 1‑36, human IFITM1 shares only 47% aa identity with mouse IFITM1. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Human IFITM1 by Western Blot. Western blot shows lysates of Jurkat human acute T cell leukemia cell line, TF‑1 human erythroleukemic cell line, human placenta tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human IFITM1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4827) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for IFITM1 at approximately 17 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8. | |
IFITM1 in K562 Human Cell Line. IFITM1 was detected in immersion fixed K562 human chronic myelogenous leukemia cell line using Goat Anti-Human IFITM1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4827) at 1.7 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to plasma membranes and cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells. |
