Human LRP-1 Cluster III Affinity Purified Polyclonal Ab (25 UG)【科瑞奥博】

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Human LRP-1 Cluster III Affinity Purified Polyclonal Ab (25 UG)

货号： AF4824-SP 基本售价： 1378.1 元规格： -

产品信息

概述
货号	AF4824-SP
别名	LRP1 Cluster III; LRP-1 Cluster III
全称	LDL Receptor-related Protein 1, Cluster III
反应种属	Human
应用	Immunocytochemistry(5-15 µg/mL)
目标/特异性	Detects human LRP-1 Cluster III in direct ELISAs. In direct ELISAs, less than 1% cross-reactivity with recombinant human LRP-1 Cluster II is observed.
使用方法	Immunocytochemistry: 5-15 µg/mL
来源	Reconstitute at 0.2 mg/mL in sterile PBS.
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	4035 (Human); 16971 (Mouse); 299858 (Rat)
纯化方式	Antigen Affinity-purified
免疫原	Chinese hamster ovary cell line CHO-derived human LRP-1 Cluster III Ser2522-Ile2941 Accession # Q07954
生物活性	Human
标记	Unconjugated
溶解方法	Reconstitute at 0.2 mg/mL in sterile PBS.
背景	LDL receptor-related protein 1 (LRP-1), also known as CD91 and the alpha 2-macroglobulin receptor, is a type I membrane protein in the LDL receptor superfamily. It is expressed on neurons, hepatocytes, adipocytes, vascular smooth muscle cells, fibroblasts, keratinocytes, macrophages, and megakaryocytes. LRP-1 is important for the clearance of a large number of circulating molecules involved in fatty acid metabolism and complexes of serine proteases with their inhibitors (1-4). LRP-1 also associates directly or through intracellular scaffold proteins with other membrane associated proteins on the same cell. This allows LRP-1 to modulate the activity or internalization of PDGF R beta, NMDA receptor subunits, TGF-beta receptors, Frizzled‑1, various integrins, and the prion protein PrP^C (1, 5‑10). Human LRP-1 is an N‑glycosylated and sialylated molecule that is cleaved in the Golgi to produce an 85 kDa transmembrane beta chain and a 515 kDa alpha chain that associates noncovalently with the beta chain but does not itself cross the membrane (11, 12). The alpha chain of LRP-1 contains 31 LDLR class A repeats, 34 LDLR class B repeats, and 22 EGF‑like repeats (13). The LDLR domains are clustered in four regions throughout the protein (13). LRP-1 Cluster III (aa 2522‑2941) contains ten LDLR-A cysteine-rich domains (14). Within this region, human LRP-1 shares 97% aa sequence identity with mouse and rat LRP-1. A soluble form of LRP-1 is shed into the serum and cerebrospinal fluid and retains ligand binding properties (15, 16). LRP-1 Cluster III contains binding sites for LRPAP/RAP (14).
运输条件	Blue Ice
存放说明	4℃
参考文献	Lillis, A.P. et al. (2008) Physiol. Rev. 88:887. Galliano, M.-F. et al. (2008) PLoS ONE 3:e2729. Bouchard, B.A. et al. (2007) J. Thromb. Haemost. 6:638. Sendra, J. et al. (2008) Cardiovasc. Res. 78:581. Muratoglu, S.C. et al. (2010) J. Biol. Chem. 258:14308. Martin, A.M. et al. (2008) J. Biol. Chem. 283:12004. Cabello-Verugio, C. and E. Brandan (2007) J. Biol. Chem. 282:18842. Zilberberg, A. et al. (2004) J. Biol. Chem. 279:17535. Taylor, D.R. and N.M. Hooper (2007) Biochem. J. 402:17. Parkyn, C.J. et al. (2007) J. Cell Sci. 121:773. Herz, J. et al. (1990) EMBO J. 9:1769. Strickland, D.K. et al. (1990) J. Biol. Chem. 265:17401. Herz, J. et al. (1988) EMBO J. 7:4119. Neels, J.G. et al. (1999) J. Biol. Chem. 274:31305. Liu, Q. et al. (2009) Mol. Neurodegener. 4:17. Gorovoy, M. et al. (2010) J. Leukoc. Biol. Jul 7 epub.

参考图片

LRP‑1 Cluster III in MG‑63 Human Cell Line. LRP‑1 Cluster III was detected in immersion fixed MG‑63 human osteosarcoma cell line using Sheep Anti-Human LRP‑1 Cluster III Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4824) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 493-conjugated Anti-Sheep IgG Secondary Antibody (green; Catalog # NL012) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.