| 货号 | AF4820-SP |
| 别名 | EC 2.7.11; EC 2.7.11.1; hMELK; HPK38; KIAA0175pEg3 kinase; maternal embryonic leucine zipper kinase; PK38; Protein kinase PK38 | 全称 | Maternal Embryonic Leucine Zipper Kinase |
| 反应种属 | Human |
| 应用 | Western Blot(1 µg/mL) Immunohistochemistry(5-15 µg/mL) |
| 目标/特异性 | Detects human MELK in Western blots. |
| 使用方法 | Western Blot: 1 µg/mL Immunohistochemistry: 5-15 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 9833 (Human) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human MELK Gln341-Ala470 Accession # Q14680 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | MELK (maternal embryonic leucine zipper kinase; also HPK38) is a member of the Snf1/AMPK family of serine/threonine kinases. It is expressed in blood mononuclear cells, stem cells and other tissues, and functions in cell cycle progression and pre-mRNA splicing. Human MELK is 651 amino acids (aa) in length and contains one protein kinase domain (aa 11‑263) and a kinase-associated (KA) 1 domain (aa 602‑651). MELK is activated upon phosphorylation of T167 and S171. There are multiple alternative splice variants. Two show the same 16 aa substitution for the N-terminal 87 and 48 aa, respectively; a third shows an alternate start site at M440, and a fourth shows complex splicing over aa 1‑392. Over aa 341‑470, human MELK shares 56% aa identity with mouse MELK. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Human MELK by Western Blot. Western blot shows lysates of Huh‑7 human hepatoma cell line, MO7e human megakaryocytic leukemic cell line, and THP‑1 human acute monocytic leukemia cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human MELK Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4820) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for MELK at approximately 74 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. | |
MELK in Human Breast Cancer Tissue. MELK was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using Sheep Anti-Human MELK Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4820) at 10 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to plasma membranes of glandular epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections. |
