| 货号 | AF4807-SP |
| 别名 | B2-1; CYTH1; cytoadhesin 1; cytohesin 1; cytohesin-1; D17S811ECYTOHESIN-1; FLJ34050; FLJ41900; homolog of secretory protein SEC7; PH, SEC7 and coiled-coil domain-containing protein 1; pleckstrin homology, Sec7 and coiled-coil domains 1; PSCD1; SEC7 homolog B2-1; SEC7 |
| 反应种属 | Human |
| 应用 | Western Blot(1 µg/mL) |
| 目标/特异性 | Detects endogenous human Cytohesin-1 in Western blots. |
| 使用方法 | Western Blot: 1 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 9267 (Human); 19157 (Mouse); 116691 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human Cytohesin-1 Met1-Tyr102 Accession # Q15438 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | Cytohesin-1, previously called B2-1 or PSCD1 (pleckstrin homology, Sec7 and coiled-coil domain-containing protein 1), is a 398 amino acid (aa), 47 kDa member of the cytohesin family of guanine nucleotide exchange factors. The Sec7 domain (aa 62‑244) binds the integrin beta 2 subunit cytoplasmic domain, activating integrins via inside-out signaling, thus implicated in chemotaxis, adhesion, and cell spreading. The region of human Cytohesin-1 used as an immunogen shares 97% and 96% aa identity with mouse and rat Cytohesin-1, and 67% and 69% aa identity with human cytohesins 2 and 3, respectively. Although ubiquitously expressed, Cytohesin-1 is primarily found in immune system cells, and was cloned from a natural killer (NK) cell minus T helper cell line Jurkat library. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Human Cytohesin‑1 by Western Blot. Western blot shows lysates of NK‑92 human natural killer lymphoma cell line and Jurkat human acute T cell leukemia cell line. PVDF membrane was probed with 1 µg/mL of Human Cytohesin‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4807) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for Cytohesin‑1 at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. |
