Human/Mouse/Rat ROCK2 Affinity Purified Polyclonal Ab (25 UG)【科瑞奥博】

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Human/Mouse/Rat ROCK2 Affinity Purified Polyclonal Ab (25 UG)

货号： AF4790-SP 基本售价： 1378.1 元规格： -

产品信息

概述
货号	AF4790-SP
别名	coiled-coil-containing protein kinase 2; EC 2.7.11; EC 2.7.11.1; KIAA0619; p164 ROCK-2; Rho kinase 2; rho-associated protein kinase 2; Rho-associated, coiled-coil containing protein kinase 2; ROCK-II; ROKalpha
全称	Rho-associated, Coiled-coil Containing Protein Kinase 2
反应种属	Human/Mouse/Rat
应用	Western Blot(1 µg/mL)
目标/特异性	Detects human, mouse and rat ROCK2 in Western blots.
使用方法	Western Blot: 1 µg/mL
来源	Reconstitute at 0.2 mg/mL in sterile PBS.
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	9475 (Human); 19878 (Mouse); 25537 (Rat)
纯化方式	Antigen Affinity-purified
免疫原	E. coli-derived recombinant human ROCK2 Ile641-Ala846 Accession # O75116
生物活性	Human, Mouse, Rat
标记	Unconjugated
溶解方法	Reconstitute at 0.2 mg/mL in sterile PBS.
背景	ROCK2/II (Rho-associated protein kinase 2; also Rho-kinase alpha ) is a member of the AGC serine/threonine protein kinase family. ROCK2 is a downstream target of activated RhoA. Its activation results in myosin phosphorylation with subsequent muscle contraction. Human ROCK2 is 1388 amino acids (aa) in length, and appears to be present as a dimer. It contains a protein kinase domain (aa 92‑354), a coiled-coil region (aa 429‑1131) and a zinc-finger region (aa 1260‑1315). Two isoforms show an alternate start site at M686, and a three aa substitution for the N-terminal 444 amino acids. Over aa 641‑846, human ROCK2 shares 94% and 96% aa identity with mouse and canine ROCK2, respectively.
运输条件	Blue Ice
存放说明	4℃
参考文献	Actin Stress Fibers and Focal Adhesions During EMT Apoptosis Intracellular Kinases Chemokine Receptor Intracellular Signaling Intracellular Signaling Molecules in Angiogenesis Nogo Proteins and Receptors Repulsive Guidance Molecules and Receptors Wnt Intracellular Signaling

参考图片

Detection of Human/Mouse/Rat ROCK2 by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line, COLO 205 human colorectal adenocarcinoma cell line, DA3 mouse myeloma cell line, CH‑1 mouse B cell lymphoma cell line, and PC‑12 rat adrenal pheochromocytoma cell line. PVDF membrane was probed with 1 µg/mL of Human/Mouse/Rat ROCK2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4790) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for ROCK2 at approximately 160 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.