| 货号 | AF4768-SP |
| 别名 | Androgen receptor-interacting protein 3; ARIP3; DAB2-interacting protein; DIP; E3 SUMO-protein ligase PIAS2; MGC102682; miz; MIZ1; Msx-interacting zinc finger protein; Msx-interacting-zinc finger; PIAS-NY protein; PIASx alpha; PIASX; PIASX-ALPHA; PIASX-BETA; Protein inhibitor of activated STAT x; protein inhibitor of activated STAT, 2; Protein inhibitor of activated STAT2; SIZ2; zinc finger, MIZ-type containing 4; ZMIZ4 | 全称 | Protein Inhibitor of Activated STAT 2 |
| 反应种属 | Human |
| 应用 | Western Blot(1 µg/mL) |
| 目标/特异性 | Detects human PIAS2 in Western blots. |
| 使用方法 | Western Blot: 1 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 9063 (Human); 17344 (Mouse) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human PIAS2 Met1-Gly550 Accession # O75928 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | PIAS2 (Protein inhibitor of activated STAT2; also DIP, MIZ1 and PIASx-beta ) is a 45‑70 kDa member of the PIAS family of molecules. In primary cells and tissues it is expressed in T and NK cells, and in testis where it likely regulates spermatogenesis. However, it is widely expressed in cultured cell lines. PIAS2 is known to inhibit STAT4-mediated gene activation, and to interact with inactive ELK‑1, promoting its activation via HDAC2 dissociation and desumoylation. Human PIAS2 is 621 amino acids (aa) in length. It contains a SAP domain (aa 11‑45), a zinc-finger region (aa 331‑408), a SUMO-binding motif (aa 467‑472) and an NLS (aa 484‑492). There are multiple splice variants. PIAS2x-beta /ARIP3 shows a 22 aa substitution for aa 551‑621, while PIAS-NY shows a 12 aa substitution for aa 1‑8, followed by a truncation after Gly401. Two others show a four aa substitution for aa 504‑621, and an alternate start site at Met10. Over aa 1‑550, human PIAS2 shares 98% aa identity with mouse PIAS2. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
|
Detection of Human PIAS2 by Western Blot. Western blot shows lysates of MCF‑7 human breast cancer cell line, HepG2 human hepatocellular carcinoma cell line, HeLa human cervical epithelial carcinoma cell line, and HEK293 human embryonic kidney cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human PIAS2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4768) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for PIAS2 at approximately 63-70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. |
