| 货号 | AF4755-SP |
| 别名 | ITRAF; I-TRAF; I-TRAFTRAF2; TRAF family member-associated NF-kappa-B activator; TRAF family member-associated NFKB activator; TRAF-interacting protein | 全称 | TRAF Family Member-Associated NFKB Activator |
| 反应种属 | Human/Mouse |
| 应用 | Western Blot(0.1 µg/mL) |
| 目标/特异性 | Detects endogenous human and mouse TANK in Western blots. |
| 使用方法 | Western Blot: 0.1 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 10010 (Human); 21353 (Mouse); 252961 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant mouse TANK Ala157-Thr448 Accession # P70347 |
| 生物活性 | Human, Mouse |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | TRAF (tumor necrosis factor receptor-associated factor) family member-associated NF-kappaB activator (TANK) maintains TRAF1, TRAF2 and TRAF3 proteins in their latent states (cytoplasmic sequestration). TANK binds to their TRAF-c domains inhibiting TRAF signaling and NF kappa B activation. TANK may function as an adaptor molecule in multiple antiviral pathways. Two transcript variants encoding different isoforms for this gene have been found. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Human/Mouse TANK by Western Blot. Western blot shows lysates of A20 mouse B cell lymphoma cell line, DA3 mouse myeloma cell line, Daudi human Burkitts lymphoma cell line, and Raji human Burkitts lymphoma cell line. PVDF membrane was probed with 0.1 µg/mL of Human/Mouse TANK Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4755) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for TANK at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. |
