| 货号 | AF4738-SP |
| 别名 | DKFZp451B1011; DKFZp686I0976; DKFZp686K0979; ILWEQ; KIAA0320; talin2; talin-2; TLN 2; TLN2 |
| 反应种属 | Human |
| 应用 | Western Blot(1 µg/mL) |
| 目标/特异性 | Detects endogenous human Talin2 in Western blots. In Western blots, no cross-reactivity to recombinant human Talin1 was detected. |
| 使用方法 | Western Blot: 1 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 83660 (Human); 70549 (Mouse); 315776 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human Talin2 Ala977-Arg1183 Accession # Q9Y4G6 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | Talin2 (talus; Latin for ankle, the shape of a focal adhesion plaque complex) is a 240 kDa cytoplasmic focal adhesion molecule. It likely forms dimers that link integrins to the actin cytoskeleton. Human Talin2 is 2542 amino acids (aa) in length. It contains one globular N‑terminal FERM domain that binds integrin cytodomains (aa 88‑406), followed by an Ala-rich region (aa 861‑1193) and a C‑terminal actin-binding domain (aa 2321‑2530). Human Talin2 has three potential variants. Two show alternate start sites at Met19 and Met1153, while a third has an alternate start site at Met1608 accompanied by a deletion of aa 1748‑1790. Over aa’s 977‑1183, human Talin2 shares 99% and 97% aa identity with canine and mouse Talin2, respectively. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Human Talin2 by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Human Talin2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4738) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Talin2 at approximately 240 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2. |
