| 货号 | AF4716-SP |
| 别名 | 1-phosphatidylinositol-4,5-bisphosphate phosphodiesterase beta-3; EC 3.1.4.11; FLJ37084; Phosphoinositide phospholipase C-beta-3; phospholipase C, beta 3 (phosphatidylinositol-specific); Phospholipase C-beta-3; PLC beta 3; PLCB3; PLC-beta-3 | 全称 | Phospholipase C beta 3 |
| 反应种属 | Human |
| 应用 | Western Blot(1 µg/mL) |
| 目标/特异性 | Detects human PLC-beta 3 in Western blots. Does not detect recombinant human (rh) PLC-beta 1, rhPLC-beta 2, or rhPLC-beta 4 in Western blots. |
| 使用方法 | Western Blot: 1 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 5331 (Human); 18797 (Mouse); 29322 (Rat) |
| 应用文献 | |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. M3 muscarinic receptor interaction with phospholipase C beta3 determines its signaling efficiency. | |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human PLC-beta 3 Lys27-Leu246 Accession # Q01970 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | The Phospholipase C family consists of 13 isozymes within six subfamilies, PLC-δ, -beta, -gamma, -epsilon, -zeta, and -eta. PLC-beta 3 (Phospholipase C beta-3) is a G-protein dependent phosphodiesterase that catalyzes the generation of inositol 1,4,5-trisphosphate (IP3) and diacylglycerol (DAG) from phosphatidylinositol 4,5-bisphosphate (IP2), an essential step in the intracellular transduction of many extracellular signals. The PLC-beta subfamily consists of 4 isozymes, beta 1‑4, which differ in their tissue distribution and their ability to be activated by G proteins. PLC-beta 1 and PLC-beta 3 are more widely expressed, whereas PLC-beta 2 is resticted to hematopoietic cells and PLC-beta 4 is limited to certain neuronal cells and the retina. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Human PLC‑ beta 3 by Western Blot. Western blot shows lysates of DU145 human prostate carcinoma cell line, HEK293 human embryonic kidney cell line, and HeLa human cervical epithelial carcinoma cell line. PVDF membrane was probed with 1 µg/mL Sheep Anti-Human PLC‑ beta 3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4716) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band for PLC‑ beta 3 was detected at approximately 150 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. | |
Detection of Human PLC‑ beta 3 by Western Blot. Western blot shows recombinant human PLC-beta 1, PLC-beta 2, PLC-beta 3, PLC-beta 4 (5 ng/lane). PVDF membrane was probed with 1 µg/mL Sheep Anti-Human PLC‑ beta 3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4716) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band for PLC‑ beta 3 was detected at approximately 29 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. |
