Human DC-SCRIPT/ZNF366 Affinity Purified Polyclonal Ab (25 UG)【科瑞奥博】

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Human DC-SCRIPT/ZNF366 Affinity Purified Polyclonal Ab (25 UG)

货号： AF4707-SP 基本售价： 1378.1 元规格： -

产品信息

概述
货号	AF4707-SP
别名	FLJ39796; zinc finger protein 366; ZNF366
全称	Dendritic Cell-specific Transcript
反应种属	Human
应用	Western Blot(0.1 µg/mL) Intracellular Staining by Flow Cytometry(2.5 µg/10⁶cells)
目标/特异性	Detects human DC-SCRIPT/ZNF366 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 1% cross-reactivity with recombinant human (rh) ZNF24 and rhZNF206 is observed.
使用方法	Western Blot: 0.1 µg/mL Intracellular Staining by Flow Cytometry: 2.5 µg/10⁶cells
来源	Reconstitute at 0.2 mg/mL in sterile PBS.
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	167465 (Human); 238803 (Mouse)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Dendritic cell-specific transcript: dendritic cell marker and regulator of TLR-induced cytokine production. Authors: Hontelez S, Ansems M, Karthaus N J. Immunol., 2012;189(1):138-45. Species: Human Sample Type: Cell Lysates Application: WB DC-SCRIPT: nuclear receptor modulation and prognostic significance in primary breast cancer. Authors: Ansems M, Hontelez S, Looman MW, Karthaus N, Bult P, Bonenkamp JJ, Jansen JH, Sweep FC, Span PN, Adema GJ J. Natl. Cancer Inst., 2010;102(1):54-68. Species: Human Sample Type: Whole Tissue Application: IHC Frozen
纯化方式	Antigen Affinity-purified
免疫原	E. coli-derived recombinant human DC-SCRIPT/ZNF366 Met559-Ile744 Accession # Q8N895
生物活性	Human
标记	Unconjugated
溶解方法	Reconstitute at 0.2 mg/mL in sterile PBS.
背景	DC-SCRIPT (dendritic cell-specific transcript; also ZNF366) is an 85 kDa member of the Cys-Cys:His-His subfamily of zinc-finger proteins. This subfamily is principally involved in protein-DNA interactions. DC-SCRIPT appears to be DC specific, and is expressed in myeloid DC, plasmacytoid DC and Langerhans cells. Human DC-SCRIPT is 744 amino acids (aa) in length. It contains an R-K-R-K NLS (aa 77-80), a Pro-rich region (aa 110-219), eleven C2H2-type zinc-fingers (aa 255‑556) and an extended acidic region (aa 586-698). Over aa 559-744, human DC-SCRIPT is 68% aa identical to mouse DC-SCRIPT.
运输条件	Blue Ice
存放说明	4℃

参考图片

Detection of DC‑SCRIPT/ZNF366 in Human Mature Dendritic Cells by Flow Cytometry. Human mature dentritic cells differentated from human peripheral blood mononuclear cell derived CD14⁺ cells treated with 20 ng/mL Recombinant Human IL‑4 (Catalog # 204-IL) and 50 ng/mL Recombinant Human GM‑CSF (Catalog # 215-GM) for 7 days and 1 μg/mL LPS, 20 ng/mL Recombinant Human TNF‑ alpha (Catalog # 210-TA), and 20 ng/mL Recombinant Human IL‑1 beta /IL‑1F2 (Catalog # 201-LB) for last 24 hours were stained with Human DC‑SCRIPT/ZNF366 Antigen Affinity‑purified Polyclonal Antibody (Catalog # AF4707, filled histogram), or control antibody (Catalog # AB-108-C, open histogram), followed by Donkey Anti-Goat IgG (H+L) Phycoerythrin (Catalog # F0107). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.