| 货号 | AF4706-SP |
| 别名 | LAT-like membrane associated protein; LAXFLJ20340; Linker for activation of X cells; lymphocyte transmembrane adapter 1; lymphocyte transmembrane adaptor 1; Membrane-associated adapter protein LAX | 全称 | Lymphocyte Transmembrane Adaptor 1 |
| 反应种属 | Human |
| 应用 | Western Blot(1 µg/mL) Intracellular Staining by Flow Cytometry(0.25 µg/106cells) |
| 目标/特异性 | Detects human LAX1 in direct ELISAs and Western blots. |
| 使用方法 | Western Blot: 1 µg/mL Intracellular Staining by Flow Cytometry: 0.25 µg/106cells |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 54900 (Human); 240754 (Mouse); 498232 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human LAX1 isoform 1 Asn136-Glu398 Accession # Q8IWV1 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | LAX1 (Linker for activation of X cells) is a 70 kDa member of a small group of T cell transmembrane (TM) adaptor proteins. It is upregulated on both T and B cells after activation, and likely downmodulates both BCR and TCR signaling. Human LAX1 is 398 amino acids (aa) in length. Although its predicted MW is 44 kDa, it runs anomalously at 70 kDa in SDS-PAGE. It is a type III (no signal sequence) TM protein that contains a 37 aa N-terminal extracellular region and a 340 aa cytoplasmic domain (aa 59‑398). There are two potential isoform variants. One has an alternate start site at Met77, while a second shows a 13 aa substitution for aa 1‑29. Over aa 136‑398, human LAX1 is 47% aa identical to mouse LAX1. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Human LAX1 by Western Blot. Western blot shows lysates of Jurkat human acute T cell leukemia cell line and K562 human chronic myelogenous leukemia cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human LAX1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4706) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for LAX1 at approximately 70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. | |
Detection of LAX1 in Jurkat Human Cell Line by Flow Cytometry. Jurkat human acute T cell leukemia cell line were stained with Goat Anti-Human LAX1 Antigen Affinity‑purified Polyclonal Antibody (Catalog # AF4706, filled histogram) or isotype control antibody (Catalog # AB-108-C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin. |
