| 货号 | AF4694-SP |
| 别名 | CORD10; RP35; Sema B; sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and shortcytoplasmic domain, (semaphorin) 4A; SEMA4A; SEMAB; semaphorin-4A; semaphorin-B; SEMB; SEMBFLJ12287 |
| 反应种属 | Human |
| 应用 | Western Blot(2 µg/mL) |
| 目标/特异性 | Detects human Semaphorin 4A in direct ELISAs and Western blots. In direct ELISAs, approximately 80% cross-reactivity with recombinant mouse Semaphorin 4A is observed, and less than 1% cross-reactivity with recombinant human (rh) Semaphorin 4B, rhSemaphorin 4C, and rhSemaphorin 4D is observed. |
| 使用方法 | Western Blot: 2 µg/mL |
| 来源 | Sterile PBS to a final concentration of 0.2 mg/mL. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 64218 (Human); 20351 (Mouse); 310630 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | Mouse myeloma cell line NS0-derived recombinant human Semaphorin 4A Gly32-His683 Accession # Q9H3S1 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Sterile PBS to a final concentration of 0.2 mg/mL. |
| 背景 | Sema4A (Semaphorin 4A; also Sema B) is a class IV member of the semaphorin family of proteins. It is expressed by dendritic cells, monocytes, T cells, B cells, astrocytes and oligodendrocytes, plus visceral smooth muscle and bronchial epithelial cells. It reportedly binds to Tim-2 and Plexin D1 and B1. In the immune system, it serves to activate naïve T cells and induce a Th1 phenotype. In the nervous system, it induces growth cone collapse in neurons. Mature human Sema4A is an 80 kDa (predicted) type I transmembrane glycoprotein that is 729 amino acids (aa) in length (aa 33-761). It contains a 651 aa extracellular region (aa 33-683) that is characterized by the presence of one Sema domain (aa 36-494), a PSI region (aa 496-528), and an Ig-like C2-type domain (aa 573-631). The 57 aa cytoplasmic region contains two potential phosphorylation sites at Thr744 and Ser747. There are two potential splice variants. One contains a 37 aa substitution for aa 293-328, while a second contains an alternative start site at Met100 coupled to a deletion of aa 122-154. Sema4A is reportedly cleaved to generate both a monomeric and a dimeric soluble isoforms. Over aa 32-683, human Sema4A shares 86% aa identity with mouse Sema4A. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Human Semaphorin 4A by Western Blot. Western blot shows lysates of human mature dendritic cells. PVDF membrane was probed with 2 µg/mL of Sheep Anti-Human Semaphorin 4A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4694) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Semaphorin 4A at approximately 90 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. |
