| 货号 | AF4688-SP |
| 别名 | ARVD11; Cadherin family member 2; CDHF2; CDHF2DGII/III; desmocollin 2; desmocollin-2; Desmocollin-3; Desmosomal glycoprotein II; desmosomal glycoprotein II/III; Desmosomal glycoprotein III; DG2; DSC2; DSC3DKFZp686I11137 |
| 反应种属 | Human |
| 应用 | Western Blot(0.1 µg/mL) Immunoprecipitation(25 µg/mL) |
| 目标/特异性 | Detects human Desmocollin-2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 5% cross-reactivity with recombinant human (rh) Desmocollin-1 and rhDesmocollin-3 is observed. |
| 使用方法 | Western Blot: 0.1 µg/mL Immunoprecipitation: 25 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 1824 (Human); 13506 (Mouse); 291760 (Rat) |
| 应用文献 | |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Membrane-impermeable cross-linking provides evidence for homophilic, isoform-specific binding of desmosomal cadherins in epithelial cells. | |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | Mouse myeloma cell line NS0-derived recombinant human Desmocollin-2 Arg136-Arg684 Accession # Q02487 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | Desmocollin-2 (DSC2) is a 100-110 kDa transmembrane glycoprotein member of the cadherin family of calcium dependent adhesion molecules (1 - 3). Human DSC2 is synthesized as a 901 amino acid (aa) precursor. It contains a 108 aa propeptide plus a mature region that consists of a 559 aa extracellular domain (ECD) that contains five cadherin-like domains, a 21 aa transmembrane segment, and a 186 aa cytoplasmic region (4). Within the ECD, human DSC2 shares 74% - 79% aa sequence identity with bovine, mouse, and rat DSC2. It shares 54% and 64% aa sequence identity with Desmocollin-1 and -3, respectively. DSC2 is also expressed as long and short splice forms which differ in their cytoplasmic regions (4, 5). The N-terminal two cadherin-like domains mediate homophilic interactions as well as heterophilic interactions with Desmoglein-2 (6). DSC2 is one of the principal components of desmosomes which form adhesive contacts between epithelial cells (1, 2). It is expressed in the basal and suprabasal layers of stratified epithelia in many tissues (1, 5, 7). In contrast to DSC1 and DSC3, DSC2 is also expressed in simple epithelia lining the gastrointestinal tract, liver, and kidney (5). During colon carcinogenesis however, DSC2 is downregulated, while DSC1 and DSC3 are upregulated (8). DSC2 is additionally expressed in the myocardium, and a variety of DSC2 mutations are associated with the cardiac arrhythmia disorder ARVC (5, 9). |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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