| 货号 | AF4680-SP |
| 别名 | CASP2 and RIPK1 domain containing adaptor with death domain; Caspase and RIP adapter with death domain; caspase and RIP adaptor with death domain; death domain containing protein CRADD; death domain-containing protein CRADD; MGC9163; RAIDD; RAIDDdeath adaptor molecule RAIDD; RIP-associated ICH1/CED3-homologous protein with death domain; RIP-associated protein with a death domain | 全称 | CASP2 and RIPK1 Domain Containing Adaptor With Death Domain |
| 反应种属 | Human/Mouse/Rat |
| 应用 | Western Blot(1 µg/mL) |
| 目标/特异性 | Detects endogenous human, mouse, and rat CRADD in Western blots. |
| 使用方法 | Western Blot: 1 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 8738 (Human); 12905 (Mouse); 314756 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant mouse CRADD Met1-Glu199 Accession # O88843 |
| 生物活性 | Human, Mouse, Rat |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | CRADD (Caspase and RIPadapter with deathdomain; also RAIDD) is a 22 kDa, widely-expressed cytosolic adaptor protein that participates in apoptosis. Mouse CRADD is 199 amino acids (aa) in length. It contains an N-terminal CARD region (aa 1‑92) and a C-terminal DD/death domain (aa 116‑188). The CARD interacts with caspase-2, while the DD recruits either PIDD or RIP. These interactions contribute to the activation of an apoptotic cascade. Mouse CRADD shows two potential additional isoforms. One shows an alternate start site at Met55, while a second shows a 70 aa substitution for aa’s 101‑199. Mouse CRADD shares 90% and 95% aa identity with human and rat CRADD, respectively. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Human/Mouse/Rat CRADD by Western Blot. Western blot shows lysates of Raji human Burkitts lymphoma cell line, A20 mouse B cell lymphoma cell line, and L6 rat myoblast cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse/Rat CRADD Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4680) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for CRADD at approximately 26 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2. |
