Human/Mouse EphB2 Affinity Purified Polyclonal Ab (25 UG)【科瑞奥博】

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Human/Mouse EphB2 Affinity Purified Polyclonal Ab (25 UG)

货号： AF467-SP 基本售价： 1378.1 元规格： -

产品信息

概述
货号	AF467-SP
别名	CAPB; Cek5; Drt; DRTEphB2; EC 2.7.10; EC 2.7.10.1; EK5; elk-related tyrosine kinase; EPH receptor B2; eph tyrosine kinase 3; EPH-like kinase 5; ephrin type-B receptor 2; EPHT3MGC87492; EPTH3; Erk; ERKHek5; Hek5; Nuk; PCBC; protein-tyrosine kinase HEK5; Qek2; Renal carcinoma antigen NY-REN-47; Sek3; Tyro5; Tyrosine-protein kinase receptor EPH-3; Tyrosine-protein kinase TYRO5
全称	Eph Receptor B2
反应种属	Human/Mouse
应用	Western Blot(0.1 µg/mL) Flow Cytometry(0.25 µg/10⁶cells) Immunohistochemistry(1-15 µg/mL) Immunocytochemistry(5-15 µg/mL)
目标/特异性	Detects mouse and human EphB2 in direct ELISAs and Western blots. In Western blots, approximately 5% cross-reactivity with recombinant rat (rr) EphB1, recombinant mouse (rm) EphA8, rmEphA6, rmEphB6, rmEphA3, rmEphA4, rmEphA7, rrEphA5, recombinant human EphA1, rmEphA2 and rmEphB3 is observed.
使用方法	Western Blot: 0.1 µg/mL Flow Cytometry: 0.25 µg/10⁶cells Immunohistochemistry: 1-15 µg/mL Immunocytochemistry: 5-15 µg/mL
来源	Reconstitute at 0.2 mg/mL in sterile PBS.
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	2048 (Human); 13844 (Mouse)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. EphrinB2 repression through ZEB2 mediates tumour invasion and anti-angiogenic resistance Nat Commun, 2016;7(0):12329. Species: Mouse Sample Type: Tissue Homogenates Application: WB Tissue-Specific Effects of Reduced beta-catenin Expression on Adenomatous Polyposis Coli Mutation-Instigated Tumorigenesis in Mouse Colon and Ovarian Epithelium. Authors: Feng Y, Sakamoto N, Wu R, Liu J, Wiese A, Green M, Green M, Akyol A, Roy B, Zhai Y, Cho K, Fearon E PLoS Genet, 2015;11(11):e1005638. Species: Mouse Sample Type: Whole Tissue Application: IHC - paraffin GluA2 trafficking is involved in apoptosis of retinal ganglion cells induced by activation of EphB/EphrinB reverse signaling in a rat chronic ocular hypertension model. Authors: Dong L, Gao F, Wang X, Miao Y, Wang S, Wu Y, Li F, Wu J, Cheng X, Sun X, Yang X, Wang Z J Neurosci, 2015;35(13):5409-21. Species: Rat Sample Type: Tissue Homogenates Application: WB Instruction of circulating endothelial progenitors in vitro towards specialized blood-brain barrier and arterial phenotypes. Authors: Boyer-Di Ponio J, El-Ayoubi F, Glacial F, Ganeshamoorthy K, Driancourt C, Godet M, Perriere N, Guillevic O, Couraud P, Uzan G PLoS ONE, 2014;9(1):e84179. Species: Human Sample Type: Whole Cells Application: ICC Fluorescence A mathematical model for eph/ephrin-directed segregation of intermingled cells. Authors: Aharon, Rotem, Janes, Peter W, Burgess, Anthony, Hamza, Kais, Klebaner, Fima, Lackmann, Martin PLoS ONE, 2014;9(12):e111803. Species: Human Sample Type: Cell Lysate Application: WB Neuropsin cleaves EphB2 in the amygdala to control anxiety. Authors: Attwood BK, Bourgognon JM, Patel S, Mucha M, Schiavon E, Skrzypiec AE, Young KW, Shiosaka S, Korostynski M, Piechota M, Przewlocki R, Pawlak R Nature, 2011;473(7347):372-5. Species: Mouse Sample Type: In Vivo Application: In vivo Smad3 contributes to positioning of proliferating cells in colonic crypts by inducing EphB receptor protein expression. Authors: Furukawa K, Sato T, Katsuno T, Nakagawa T, Noguchi Y, Tokumasa A, Yokote K, Yokosuka O, Saito Y Biochem. Biophys. Res. Commun., 2011;405(4):521-6. Species: Mouse Sample Type: Cell Lysates Application: WB Ephrin-B1 regulates axon guidance by reverse signaling through a PDZ-dependent mechanism. Authors: Bush JO, Soriano P Genes Dev., 2009;23(13):1586-99. Species: Mouse Sample Type: Tissue Homogenates Application: IP EphrinB2 regulation by PTH and PTHrP revealed by molecular profiling in differentiating osteoblasts. Authors: Allan EH, Hausler KD, Wei T, Gooi JH, Quinn JM, Crimeen-Irwin B, Pompolo S, Sims NA, Gillespie MT, Onyia JE, Martin TJ J. Bone Miner. Res., 2008;23(8):1170-81. Species: Mouse Sample Type: Cell Lysates Application: WB Ephrin-B reverse signaling promotes structural and functional synaptic maturation in vivo. Authors: Lim BK, Matsuda N, Poo MM Nat. Neurosci., 2008;11(2):160-9. Species: Xenopus Sample Type: Tissue Homogenates Application: IP Phosphorylation of ephrin-B1 regulates dissemination of gastric scirrhous carcinoma. Authors: Tanaka M, Kamata R, Takigahira M, Yanagihara K, Sakai R Am. J. Pathol., 2007;171(1):68-78. Species: Mouse Sample Type: Whole Cells Application: WB Cupredoxin-cancer interrelationship: azurin binding with EphB2, interference in EphB2 tyrosine phosphorylation, and inhibition of cancer growth. Authors: Chaudhari A, Mahfouz M, Fialho AM, Yamada T, Granja AT, Zhu Y, Hashimoto W, Schlarb-Ridley B, Cho W, Das Gupta TK, Chakrabarty AM Biochemistry, 2007;46(7):1799-810. Species: Human Sample Type: Cell Lysates Application: IP Fish oil increases cholesterol storage in white adipose tissue with concomitant decreases in inflammation, hepatic steatosis, and atherosclerosis in mice. Authors: Saraswathi V, Gao L, Morrow JD, Chait A, Niswender KD, Hasty AH J. Nutr., 2007;137(7):1776-82. Species: Human Sample Type: Whole Tissue Application: IHC Paraffin-embedded EphB receptors coordinate migration and proliferation in the intestinal stem cell niche. Authors: Holmberg J, Genander M, Halford MM, Anneren C, Sondell M, Chumley MJ, Silvany RE, Henkemeyer M, Frisen J Cell, 2006;125(6):1151-63. Species: Mouse Sample Type: Tissue Homogenates Application: IP EphB2 and ephrin-B1 expressed in the adult kidney regulate the cytoarchitecture of medullary tubule cells through Rho family GTPases. Authors: Ogawa K, Wada H, Okada N, Harada I, Nakajima T, Pasquale EB, Tsuyama S J. Cell. Sci., 2006;119(0):559-70. Species: Mouse Sample Type: Whole Tissue Application: IHC EphB2/R-Ras signaling regulates glioma cell adhesion, growth, and invasion. Authors: Nakada M, Niska JA, Tran NL, McDonough WS, Berens ME Am. J. Pathol., 2005;167(2):565-76. Species: Human Sample Type: Cell Lysates Application: WB The phosphorylation of EphB2 receptor regulates migration and invasion of human glioma cells. Authors: Nakada M, Niska JA, Miyamori H, McDonough WS, Wu J, Sato H, Berens ME Cancer Res., 2004;64(9):3179-85. Species: Human Sample Type: Cell Lysates Application: IP
纯化方式	Antigen Affinity-purified
免疫原	Mouse myeloma cell line NS0-derived recombinant mouse EphB2 Val27-Lys548 Accession # P54763
生物活性	Human, Mouse
标记	Unconjugated
溶解方法	Reconstitute at 0.2 mg/mL in sterile PBS.
背景	EphB2, also known as Cek5, Nuk, Erk, Qek2, Tyro5, Sek3, Hek5, and Drt (1), is a member of the Eph receptor family which binds members of the ephrin ligand family. There are two classes of receptors, designated A and B. Both the A and B class receptors have an extracellular region consisting of a globular domain, a cysteine-rich domain, and two fibronectin type III domains. This is followed by the transmembrane region and the cytoplasmic region. The cytoplasmic region contains a juxtamembrane motif with two tyrosine residues which are the major autophosphorylation sites, a kinase domain, and a conserved sterile alpha motif (SAM) in the carboxy tail which contains one conserved tyrosine residue. Activation of kinase activity occurs after ligand recognition and binding. EphB2 has been shown to bind ephrin-B1, ephrin-B2, and ephrin-B3 (2, 3). The extracellular domains of human and mouse EphB2 share 99% amino acid identity. Only membrane-bound or Fc‑clustered ligands are capable of activating the receptor in vitro. Soluble monomeric ligands bind the receptor but do not induce receptor autophosphorylation and activation (2). In vivo, the ligands and receptors display reciprocal expression (3). It has been found that nearly all the receptors and ligands are expressed in developing and adult neural tissue (3). The ephrin/Eph families also appear to play a role in angiogenesis (3).
运输条件	Blue Ice
存放说明	4℃
参考文献	Eph Nomenclature Committee [letter] (1997) Cell 90:403. Flanagan, J.G. and P. Vanderhaeghen (1998) Annu. Rev. Neurosci. 21:309. Pasquale, E.B. (1997) Curr. Opin. Cell Biol. 9:608.

参考图片

Detection of EphB2 in COLO 205 Human Cell Line by Flow Cytometry. COLO 205 human colorectal adenocarcinoma cell line was stained with Goat Anti-Human/Mouse EphB2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF467, filled histogram) or isotype control antibody (Catalog #
AB-108-C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107). View our protocol for Staining Membrane-associated Proteins.

Detection of EphB2 in D3 Mouse Cell Line by Flow Cytometry. D3 mouse embryonic stem cell line was stained with Goat Anti-Human/Mouse EphB2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF467, filled histogram) or isotype control antibody (Catalog #
AB-108-C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107). View our protocol for Staining Membrane-associated Proteins.

EphB2 in MBA‑MB‑468 Human Cell Line. EphB2 was detected in immersion fixed MBA‑MB‑468 human breast cancer cell line using Goat Anti-Human/Mouse EphB2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF467) at 5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

EphB2 in Embryonic Mouse Brain. EphB2 was detected in immersion fixed frozen sections of embryonic mouse brain (15 d.p.c.) using 15 µg/mL Goat Anti-Mouse EphB2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF467) overnight at 4 °C. Tissue was stained with the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained (green). View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.

EphB2 in Human Esophageal Squamous Cell Carcinoma. EphB2 was detected in immersion fixed paraffin-embedded sections of human esophageal squamous cell carcinoma using Goat Anti-Human/Mouse EphB2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF467) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in cancer cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

EphB2 in Mouse Embryo. EphB2 was detected in immersion fixed frozen sections of mouse embryo (13 d.p.c.) using Goat Anti-Human/Mouse EphB2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF467) at 1.7 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to developing brain. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.