| 货号 | AF4679-SP |
| 别名 | DFF1; DFF1DNA fragmentation factor 45 kDa subunit; DFF45DNA fragmentation factor subunit alpha; DFF-45DNA fragmentation factor, 45 kD, alpha polypeptide; DFFA; DNA fragmentation factor, 45kDa, alpha polypeptide; ICAD; Inhibitor of CAD | 全称 | DNA Fragmentation Factor, Subunit alpha |
| 反应种属 | Human |
| 应用 | Western Blot(0.5 µg/mL) |
| 目标/特异性 | Detects endogenous human DFF45 in Western blots. |
| 使用方法 | Western Blot: 0.5 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 1676 (Human); 13347 (Mouse); 114214 (Rat) |
| 应用文献 | |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Intracellular K(+) inhibits apoptosis by suppressing the Apaf-1 apoptosome formation and subsequent downstream pathways but not cytochrome c release. | |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human DFF45 Met1-Thr331 Accession # O00273 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | DFF45 (DNA fragmentation factor 45 kDa subunit, also known as ICAD) is a 45 kDa member of the CIDE domain-containing family of proteins. Human DFF45 is 331 amino acids (aa) in length. It contains an N-terminal CIDE domain (aa 17‑96) plus two caspase-3 cleavage sites (aa’s 117‑118 and 224‑225). DFF45 is a cytoplasmic protein that inhibits DFF40 DNAase. Normally, DFF45 and DFF40 form an enzymatically-inactive noncovalent heterodimer. Upon activation of the apoptotic cascade, DFF45 is cleaved into three fragments. These dissociate from DFF40 and induce DFF40 oligomerization and activation. Human DFF45 has two potential splice forms. One is truncated, and shows a seven aa substitution for aa 262‑331; a second is extended, and shows a 45 aa substitution for the C-terminal five amino acids. Human DFF45 is 76% aa identical to mouse DFF45. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
|
Detection of Human DFF45/ICAD by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 0.5 µg/mL of Human DFF45/ICAD Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4679) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for DFF45/ICAD at approximately 45 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 2. |
