| 货号 | AF4678-SP |
| 别名 | adapter protein; FLJ33531; IL-1RAcPIP; toll interacting protein; toll-interacting protein | 全称 | Toll Interacting Protein |
| 反应种属 | Human |
| 应用 | Western Blot(1 µg/mL) |
| 目标/特异性 | Detects endogenous human Tollip in Western blots. |
| 使用方法 | Western Blot: 1 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 54472 (Human); 54473 (Mouse); 361677 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human Tollip Met1-Pro274 Accession # Q9H0E2 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | Tollip (Toll-interacting protein) is a 30 kDa, cytoplasmic protein that is involved in IL-1 and Toll receptor signaling. It is classified as an adaptor protein that performs multiple functions. It binds both IRAK and Toll receptors, inhibiting constitutive IL-1 and Toll receptor signaling. It also binds to ubiquinated IL-1 RI, directing it to the endosomal degradation compartment. Human Tollip is 274 amino acids (aa) in length. It contains a C2 Ca++-binding region (aa 54‑154) and a CUE domain (aa 229‑270) that binds ubiquitin. One potential splice form exists that shows a deletion of aa’s 6‑33. Human Tollip is 92% identical to mouse and canine Tollip. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Human Tollip by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line and LNCaP human prostate cancer cell line. PVDF membrane was probed with 1 µg/mL of Human Tollip Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4678) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Tollip at approximately 30 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 4. |
