| 货号 | AF4668-SP |
| 别名 | GLUR3; glutamate receptor, metabotropic 3; GPRC1C; GPRC1Cglutamate metabotropic receptor 3; GRM3; mGlu3; mGluR3; MGLUR3metabotropic glutamate receptor 3 | 全称 | Metabotropic Glutamate Receptor 3 |
| 反应种属 | Human |
| 应用 | Western Blot(0.1 µg/mL) Immunohistochemistry(5-15 µg/mL) |
| 目标/特异性 | Detects human mGluR3 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 10% cross-reactivity with recombinant human (rh) mGluR2, rhmGluR4, rhmGluR8 is observed, and less than 1% cross-reactivity with rhmGluR1, rhmGluR5, and rhmGluR7 is observed. |
| 使用方法 | Western Blot: 0.1 µg/mL Immunohistochemistry: 5-15 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 2913 (Human); 108069 (Mouse); 24416 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | Chinese hamster ovary cell line CHO-derived recombinant human mGluR3 Asp25-Ser507 Accession # Q14832 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | Metabotropic glutamate receptor 3 (mGluR3) is a 90-100 kDa, 7-transmembrane glycoprotein that belongs to group II of the C-family of G-protein coupled receptors. It is a presynaptic receptor expressed on both neurons and glia, whose activation reduces adenylate cyclase activity. Mature human mGluR3 is 857 amino acids in length and contains a 554 amino acid (aa) N-terminal extracellular domain (ECD) (aa 23-576). The ECD binds glutamate and forms homodimers. There is one alternative splice form that is soluble, 515 aa in length and shows a 96 aa substitution for aa 442-879. Over aa 25-507, human mGluR3 shares 97% aa sequence identity with mouse and rat mGluR3 and 67% aa sequence identity with hGluR2. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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mGluR3 in Human Brain. mGluR3 was detected in immersion fixed paraffin-embedded sections of human brain (caudate nucleus) using Sheep Anti-Human mGluR3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4668) at 10 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific labeling was localized to the plasma membrane and cytoplasm of neurons. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections. |
