| 货号 | AF4660-SP |
| 别名 | BHLHB26; bHLHb26cell growth-inhibiting gene 8; Class B basic helix-loop-helix protein 26; DNA-binding protein inhibitor ID2; DNA-binding protein inhibitor ID-2; GIG8; helix-loop-helix protein ID2; ID2A; ID2H; inhibitor of differentiation 2; Inhibitor of DNA binding 2; inhibitor of DNA binding 2, dominant negative helix-loop-helix protein; MGC26389 | 全称 | Inhibitor of DNA Binding 2 |
| 反应种属 | Human |
| 应用 | Western Blot(1 µg/mL) Immunocytochemistry(5-15 µg/mL) |
| 目标/特异性 | Detects human ID2 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human (rh) ID1, rhID3, and rhID4 is observed. |
| 使用方法 | Western Blot: 1 µg/mL Immunocytochemistry: 5-15 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 3398 (Human); 15902 (Mouse); 25587 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human ID2 Met1-Gly134 Accession # Q02363 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | ID2 (Inhibitor of DNA binding 2; also known as inhibitor of differentiation 2) is a 15 kDa member of the small ID HLH family of proteins. It is a nuclear protein that forms inactivating heterodimers with bHLH transcription factors. bHLH proteins activate lineage-specific genes and ID2. Human ID2 is 134 amino acids (aa) in length. It contains one HLH domain (aa 36-76) but no DNA-binding motif. Full-length human ID2 is 98% aa identical to both mouse and porcine ID2. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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ID2 in A549 Human Cell Line. ID2 was detected in immersion fixed A549 human lung carcinoma cell line using 10 µg/mL Human ID2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4660) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red, upper panel; Catalog # NL001) and counterstained with DAPI (blue, lower panel). View our protocol for Fluorescent ICC Staining of Cells on Coverslips. | |
Detection of Human ID2 by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Human ID2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4660) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for ID2 at approximately 15 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8. |
