Human Dermatopontin Affinity Purified Polyclonal Ab (25 UG)【科瑞奥博】

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Human Dermatopontin Affinity Purified Polyclonal Ab (25 UG)

货号： AF4629-SP 基本售价： 1378.1 元规格： -

产品信息

概述
货号	AF4629-SP
别名	dermatopontin; DPT; Eq1; TRAMP; Tyrosine-rich acidic matrix protein
反应种属	Human
应用	Western Blot(1 µg/mL)
目标/特异性	Detects human Dermatopontin in direct ELISAs and Western blots. In direct ELISAs, approximately 50% cross-reactivity with recombinant mouse Dermatopontin is observed.
使用方法	Western Blot: 1 µg/mL
来源	Sterile PBS to a final concentration of 0.2 mg/mL.
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	1805 (Human); 56429 (Mouse); 289178 (Rat)
纯化方式	Antigen Affinity-purified
免疫原	Mouse myeloma cell line NS0-derived recombinant human Dermatopontin Gln19-Val201 Accession # AAH33736
生物活性	Human
标记	Unconjugated
溶解方法	Sterile PBS to a final concentration of 0.2 mg/mL.
背景	Dermatopontin, also known as TRAMP (tyrosine rich acidic matrix protein), is a widely expressed noncollagenous protein component of the extracellular matrix (1, 2). Mature human Dermatopontin shares 96%, 92%, and 92% amino acid sequence identity with bovine, mouse, and rat Dermatopontin, respectively. It is a 22 kDa molecule that is tyrosine sulfated but not glycosylated (3, 4). Dermatopontin contains three disulfide bonded loop structures that enclose conserved hexapeptide motifs (5). It accelerates collagen fibril formation in vitro, and Dermatopontin deficient mice exhibit altered collagen matrix deposition and organization (6 - 8). Dermatopontin is downregulated in fibrotic growths such as leiomyoma and scar tissue (9, 10). It binds both TGF-beta and the proteoglycan decorin, interactions that can increase the bioavailability of TGF-beta (11, 12). Dermatopontin promotes bone mineralization under the control of the vitamin D receptor and inhibits BMP-2 effects on osteoblast precursors (13, 14).
运输条件	Blue Ice
存放说明	4℃
参考文献	Okamoto, O. and S. Fujiwara (2006) Connect. Tissue Res. 47:177. Superti-Furga, A. et al. (1993) Genomics 17:463. Forbes, E.G. et al. (1994) FEBS Lett. 351:433. Cronshaw, A.D. et al. (1993) Matrix 13:255. Neame, P.J. et al. (1989) J. Biol. Chem. 264:5474. MacBeath, J.R.E. et al. (1993) J. Biol. Chem. 268:19826. Takeda, U. et al. (2002) J. Invest. Dermatol. 119:678. Cooper, L.J. et al. (2006) Invest. Opthalmol. Vis. Sci. 47:3303. Catherino, W.H. et al. (2004) Genes Chromosomes Cancer 40:204. Kuroda, K. et al. (1999) J. Invest. Dermatol. 112:706. Okamoto, O. et al. (1996) J. Biochem. 119:106. Okamoto, O. et al. (1999) Biochem. J. 337:537. Pochampally, R.R. et al. (2007) J. Bone Miner. Res. 22:1338. Behnam, K. et al. (2006) Connect. Tissue Res. 47:271.

参考图片

Detection of Human Dermatopontin by Western Blot. Western blot shows lysates of human heart tissue. PVDF Membrane was probed with 1 µg/mL of Human Dermatopontin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4629) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Dermatopontin at approximately 22 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.