Human PKC delta Affinity Purified Polyclonal Ab (25 UG)【科瑞奥博】

产品中心

当前位置：首页>产品中心

Human PKC delta Affinity Purified Polyclonal Ab (25 UG)

货号： AF4585-SP 基本售价： 1378.1 元规格： -

产品信息

概述
货号	AF4585-SP
别名	EC 2.7.11; EC 2.7.11.13; MAY1; MGC49908; nPKC-delta; PKCD; PRKCD; protein kinase C delta type; protein kinase C delta VIII; protein kinase C, delta
全称	Protein Kinase C delta
反应种属	Human
应用	Western Blot(1 µg/mL)
目标/特异性	Detects human PKCδ in Western blots.
使用方法	Western Blot: 1 µg/mL
来源	Reconstitute at 0.2 mg/mL in sterile PBS.
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	5580 (Human)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. p63 and p73, members of the p53 gene family, transactivate PKCdelta. Authors: Ponassi, Raffaell, Terrinoni, Alessand, Chikh, Anissa, Rufini, Alessand, Lena, Anna Mar, Sayan, Berna S, Melino, Gerry, Candi, Eleonora Biochem Pharmacol, 2006;72(11):1417-22. Species: Human Sample Type: Cell Lysates Application: WB
纯化方式	Antigen Affinity-purified
免疫原	E. coli-derived recombinant human PKCδ His549-Asp676 Accession # Q05655
生物活性	Human
标记	Unconjugated
溶解方法	Reconstitute at 0.2 mg/mL in sterile PBS.
背景	PKCδ (protein kinase C-delta) is a 78 kDa member of the PKC subfamily, AGC Ser/Thr protein kinase family of enzymes. It is a Ca⁺⁺‑independent and phospholipid‑dependent ubiquitous enzyme that participates in cellular apoptosis. Human PKCδ is 676 amino acids (aa) in length. It contains two general regions: a phosphotyrosine and DAG-type 2 binding region (aas 1‑90 and 159‑208, respectively), and an ATP-binding catalytic region (aa 349‑603). Functionally, inactive cytoplasmic PKCδ is first activated by Tyr64 and Tyr155 phosphorylation. This induces PKCδ entry into the nucleus, where it is cleaved into 43 kDa and 36 kDa fragments, initiating apoptosis. There are two potential alternate start sites for PKCδ at Met51 and Met74. In some cells, a poorly characterized 160 kDa form of PKCδ has been noted. Over aa 549‑676, human PKCδ is 85% aa identical to mouse PKCδ.
运输条件	Blue Ice
存放说明	4℃
参考文献	Chemokine Receptor Intracellular Signaling C-type Lectin Receptors Diabetes Intracellular Kinases Intracellular Kinases in the Akt Pathway Intracellular Signaling Molecules in Angiogenesis PDGF Family Positive Regulators of the Jak/STAT Pathway TNF Superfamily Regulators VEGF Family Wnt Intracellular Signaling

参考图片

Detection of Human PKCδ by Western Blot. Western blot shows lysates of LNCaP human prostate cancer cell line and HT‑29 human colon adenocarcinoma cell line. PVDF Membrane was probed with 1 µg/mL of Sheep Anti-Human PKCδ Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4585) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for PKCδ at approximately 78 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.