| 货号 | AF4559-SP |
| 别名 | EC 2.7.11; EC 2.7.11.25; MAP3K11; MEKK11; mitogen-activated protein kinase kinase kinase 11; Mixed lineage kinase 3; MLK3MLK-3; PTK1; PTK1protein-tyrosine kinase PTK1; RHOE; SH3 domain-containing proline-rich kinase; SPRK; SPRKMGC17114; Src-homology 3 domain-containing proline-rich kinase | 全称 | Mixed Lineage Kinase 3 |
| 反应种属 | Human |
| 应用 | Western Blot(1 µg/mL) |
| 目标/特异性 | Detects endogenous human MLK3 in Western blots. |
| 使用方法 | Western Blot: 1 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 4296 (Human); 26403 (Mouse); 309168 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human MLK3 Ala85-Gly207 Accession # Q16584 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | Mixed-lineage kinases (MLKs) function as mitogen-activated protein kinase kinase kinases (MAP3Ks), activating the MAPK signaling cascades. MLK3, also known as MAP3K11 and PTK1, encodes a protein of 847 amino acids (aa) that shares an unusual leucine zipper‑basic motif found in other MLKs. Small interfering RNA experiments have indicated that MLK3 is required for both mitogen and cytokine activation of JNK1, p38 alpha, and ERK1. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Human MLK3 by Western Blot. Western blot shows lysates of Ramos human Burkitts lymphoma cell line. PVDF membrane was probed with 1 µg/mL of Human MLK3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4559) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for MLK3 at approximately 93 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 1. |
