| 货号 | AF4558-SP |
| 别名 | actin filament associated protein 1; AFAP1; AFAP-110; FLJ56849 | 全称 | Actin Filament-associated Protein, 110 kDa |
| 反应种属 | Human |
| 应用 | Western Blot(1 µg/mL) |
| 目标/特异性 | Detects endogenous human AFAP in Western blots. |
| 使用方法 | Western Blot: 1 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 60312 (Human); 70292 (Mouse) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human AFAP His450-Thr730 Accession # Q8N556 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | AFAP (actin filament associated protein; also known as AFAP-110) is an adaptor protein that regulates actin filament integrity. It is both multimodular and multimeric, and is known to simultaneously bind actin and cytosolic kinases. As a homomultimer, AFAP binds and separates actin filaments, preventing actin cross-linking. Upon binding to kinases, AFAP is destabilized, promoting actin polymerization. Human AFAP is 730 amino acids (aa) in length, and contains SH3 and SH2 binding motifs (aa 69‑74 and aa 91‑96), two pleckstrin homology domains (aa 153‑248 and aa 347‑450) and a Leu-zipper/actin binding region (aa 511‑637). Over aa 450‑730, human AFAP is 86% identical to mouse and rat AFAP. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Human AFAP by Western Blot. Western blot shows lysates of MCF-7 human breast cancer cell line. PVDF membrane was probed with 1 µg/mL of Human AFAP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4558) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for AFAP at approximately 110 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 1. |
