| 货号 | AF4549-SP |
| 别名 | EC 2.7.11; FLJ90748; KRS1; Mammalian STE20-like protein kinase 2; Mess1; MST-2; MST2EC 2.7.11.1; MST3; serine/threonine kinase 3 (STE20 homolog, yeast); serine/threonine kinase 3 (Ste20, yeast homolog); serine/threonine kinase 3; serine/threonine-protein kinase 3; Serine/threonine-protein kinase Krs-1; STE20-like kinase MST2; STK3 |
| 反应种属 | Human/Mouse/Rat |
| 应用 | Western Blot(1 µg/mL) |
| 目标/特异性 | Detects endogenous human, mouse and rat MST2 in Western blots. In Western blots, this antibody shows no cross‑reactivity with recombinant human MST1/STK4. |
| 使用方法 | Western Blot: 1 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 6788 (Human); 56274 (Mouse) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human MST2 Ala339-Asp456 Accession # Q13188 |
| 生物活性 | Human, Mouse, Rat |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | MST2 (mammalian sterile twenty-like 2; also known as STK3 and Krs-1) is a 58 kDa member of the GCKII group of the STE20 subfamily of the STE Ser/Thr kinase protein family. In human, MST2 is 491 amino acids (aa) in length. It contains one kinase domain (aa 27‑278), two NESs (aa 361‑371 and 438‑447) and coiled-coil domains (aa 287‑328 and 442‑475), and one NLS (aa 473‑487). A caspase-cleavage site exists between Asp322‑Ser323. MST2 is normally inactive in the cytoplasm bound to Raf-1. Cell activation induces Raf-1:MST2 dissociation, MST2 cleavage, and N-terminal translocation to the nucleus. Human MST2 is 99% and 96% aa identical to canine and mouse MST2, respectively. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Human/Mouse/Rat MST2/STK3 by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, MCF‑7 human breast cancer cell line, NIH‑3T3 mouse embryonic fibroblast cell line, and L6 rat myoblast cell line. PVDF membrane was probed with 1 µg/mL of Human/Mouse/Rat MST2/STK3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4549) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for MST2/STK3 at approximately 65 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2. |
