| 货号 | AF4548-SP |
| 别名 | BING2; CENP-C binding protein; DAP6; DAP6MGC126245; Daxx; death domain-associated protein 6; death-associated protein 6; death-domain associated protein; EAP1; ETS1-associated protein 1; Fas death domain-associated protein; Fas-binding protein; hDaxx; MGC126246 | 全称 | Fas Death Domain-associated Protein |
| 反应种属 | Human |
| 应用 | Western Blot(1 µg/mL) |
| 目标/特异性 | Detects endogenous human Daxx in Western blots. Detection of both reported Daxx isoforms is expected based on immunogen sequence. |
| 使用方法 | Western Blot: 1 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 1616 (Human); 13163 (Mouse); 140926 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human Daxx Met497-Gly626 Accession # Q9UER7 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | Daxx (Fas death domain-associated-xx; also death domain-associated protein 6) is a ubiquitously expressed, proapoptotic protein that belongs to the Daxx family of molecules. Although it has a predicted MW of 81 kDa, it runs anomously at 110 kDa in SDS-PAGE. Daxx is found in the nucleus where it represses transcription. In the cytoplasm, it binds to ASK1, where it mediates Fas-induced apoptosis. Human Daxx is 740 amino acids (aa) in length and contains three coiled-coil regions (aa 180‑217; 358‑399; 430‑489), two NLSs, and an Asp/Glu-rich acidic region (aa 434‑572). One splice variant is known that shows a 15 aa substitution for aa 696‑740. Human Daxx shares 71% and 90% aa identity with mouse and dog Daxx, respectively. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Human Daxx by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, Ramos human Burkitts lymphoma cell line, MCF-7 human breast cancer cell line, and Jurkat human acute T cell leukemia cell line. PVDF membrane was probed with 1 µg/mL of Human Daxx Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4548) followed by HRP‑conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Daxx at approximately 115 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 2. |
