| 货号 | AF4536-SP |
| 别名 | calcium/calmodulin-dependent protein kinase kinase 1; calcium/calmodulin-dependent protein kinase kinase 1, alpha; Calcium/calmodulin-dependent protein kinase kinase alpha; CaM-kinase IV kinase; CaM-kinase kinase 1; CaM-kinase kinase alpha; CaM-KK 1; CAMKK alpha protein; CaMKK alpha; CaM-KK alpha; CaMKK1; CAMKKACaMKK 1; DKFZp761M0423; EC 2.7.11; EC 2.7.11.17; MGC34095 | 全称 | Calcium/Calmodulin-dependent Protein Kinase Kinase alpha |
| 反应种属 | Human/Rat |
| 应用 | Western Blot(1 µg/mL) Simple Western(10 µg/mL) |
| 目标/特异性 | Detects endogenous human and rat CaMKK alpha in Western blots. In Western blots, this antibody does not cross-react with recombinant human CaMKK beta. |
| 使用方法 | Western Blot: 1 µg/mL Simple Western: 10 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 84254 (Human); 55984 (Mouse); 60341 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human CaMKK alpha Met1-Ser132 Accession # Q8N5S9 |
| 生物活性 | Human, Rat |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | The calcium/calmodulin (CaM) kinase signaling cascade includes the upstream kinases CaM kinase kinase alpha (CaMKK alpha ; also known as CaMKK1) and CaMKK beta (CaMKK2), and their substrates, the output kinases CaMKI and CaMKIV. Largely by activation of the transcription factor CREB, the CaM kinase cascade is implicated in neuronal development and long-term memory formation. In addition, CaMKK alpha is known to modulate skeletal muscle glucose uptake, and is upregulated during retinoic acid-induced neutrophil maturation. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Human/Rat CaMKK alpha by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, CCD-1070Sk human foreskin fibroblast cell line, K562 human chronic myelogenous leukemia cell line, and human brain tissue. PVDF membrane was probed with 1 µg/mL of Human/Rat CaMKK alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4536) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for CaMKK alpha at approximately 68 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 1. | |
Detection of Human CaMKK alpha by Simple WesternTM. Simple Western lane view shows lysates of HeLa human cervical epithelial carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for CaMKK alpha at approximately 68 kDa (as indicated) using 10 µg/mL of Sheep Anti-Human/Rat CaMKK alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4536) followed by 1:50 dilution of HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system. |
