| 货号 | AF4509-SP |
| 别名 | bladder cancer related protein XHL; T-box 3; T-box protein 3; T-box transcription factor TBX3; TBX3-ISO; ulnar mammary syndrome; UMS; XHL | 全称 | T-box Transcription Factor 3 |
| 反应种属 | Human |
| 应用 | Western Blot(1 µg/mL) Immunocytochemistry(5-15 µg/mL) |
| 目标/特异性 | Detects human TBX3 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human (rh) TBX2, rhTBX6, and rhTBX20 is observed. |
| 使用方法 | Western Blot: 1 µg/mL Immunocytochemistry: 5-15 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 6926 (Human); 21386 (Mouse); 353305 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human TBX3 Ser321-Thr483 Accession # O15119 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | TBX3 belongs to the T-box family of transcription factors, which are involved in regulation of developmental processes. TBX3 acts as a transcriptional repressor regulating anterior/posterior axis of the forelimb. Mutations in TBX3 are associated with ulnar-mammary syndrome, affecting limb, apocrine gland, tooth, hair, and genital development. TBX3 has 3 splice isoforms. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Human TBX3 by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and Daudi human Burkitts lymphoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human TBX3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4509) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for TBX3 at approximately 80 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3. |
