Human/Mouse/Rat PTK7/CCK4 Affinity Purified Polyclonal Ab (25 UG)【科瑞奥博】

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Human/Mouse/Rat PTK7/CCK4 Affinity Purified Polyclonal Ab (25 UG)

货号： AF4499-SP 基本售价： 1378.1 元规格： -

产品信息

概述
货号	AF4499-SP
别名	CCK4; CCK-4; CCK4Colon carcinoma kinase 4; colon carcinoma kinase-4; Protein-tyrosine kinase 7; Pseudo tyrosine kinase receptor 7; PTK7 protein tyrosine kinase 7; Tyrosine-protein kinase-like 7
全称	Protein Tyrosine Kinase 7
反应种属	Human/Mouse/Rat
应用	Western Blot(1 µg/mL) Simple Western(10 µg/mL)
目标/特异性	Detects human, mouse and rat PTK7/CCK4 in Western blots.
使用方法	Western Blot: 1 µg/mL Simple Western: 10 µg/mL
来源	Reconstitute at 0.2 mg/mL in sterile PBS.
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	5754 (Human); 71461 (Mouse); 301242 (Rat)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Insights into ectodomain shedding and processing of protein-tyrosine pseudokinase 7 (PTK7). Authors: Golubkov V, Strongin A J Biol Chem, 2012;287(50):42009-18. Species: Human Sample Type: Cell Culture Supernates Application: WB Potential relation of aberrant proteolysis of human protein tyrosine kinase 7 (PTK7) chuzhoi by membrane type 1 matrix metalloproteinase (MT1-MMP) to congenital defects. Authors: Golubkov VS, Aleshin AE, Strongin AY J. Biol. Chem., 2011;286(23):20970-6. Species: Human Sample Type: Whole Cells Application: ICC Flt-1 regulates vascular endothelial cell migration via a protein tyrosine kinase-7-dependent pathway. Authors: Lee HK, Chauhan SK, Kay E Blood, 2011;117(21):5762-71. Species: Human Sample Type: Cell Lysates Application: WB
纯化方式	Antigen Affinity-purified
免疫原	E. coli-derived recombinant human PTK7/CCK4 Ala31-Ser199 Accession # Q13308
生物活性	Human, Mouse, Rat
标记	Unconjugated
溶解方法	Reconstitute at 0.2 mg/mL in sterile PBS.
背景	CCK4 (colon carcinoma kinase 4, also known as PTK7) is a receptor tyrosine kinase (RTK) involved in colon carcinoma development and/or proliferation. Similar to the RTK HER3, CCK4 contains a catalytically inactive tyrosine kinase domain, suggesting a potential tumor-characteristic role as a signal amplifier or modulator for an as yet unidentified kinase-competent partner.
运输条件	Blue Ice
存放说明	4℃
参考文献	Cancer Biomarkers ICC / IHC Images: PTK7/CCK4 Receptor Tyrosine Kinases (RTKs) Receptor Tyrosine Kinases (RTKs) in the Akt Pathway Wnt Receptors

参考图片

Detection of Human, Mouse, and Rat PTK7/CCK4 by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, Rat-2 rat embryonic fibroblast cell line, and PT18 mouse mast/basophil cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse/Rat PTK7/CCK4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4499) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for PTK7/CCK4 at approximately 160 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 1.

Detection of Human PTK7/CCK4 by Simple Western^TM. Simple Western lane view shows lysates of HeLa human cervical epithelial carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for PTK7/CCK4 at approximately 171 kDa (as indicated) using 10 µg/mL of Goat Anti-Human/Mouse/Rat PTK7/CCK4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4499) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the
12-230 kDa separation system.