| 货号 | AF4498-SP |
| 别名 | G protein-coupled receptor kinase 1rhodopsin kinase; GPRK1; RHOK; RHOKEC 2.7.11; RKEC 2.7.11.14 | 全称 | G protein-coupled Receptor Kinase 1 |
| 反应种属 | Human/Mouse/Rat |
| 应用 | Western Blot(1 µg/mL) |
| 目标/特异性 | Detects endogenous human, mouse, and rat GRK1 in Western blots. |
| 使用方法 | Western Blot: 1 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 6011 (Human); 24013 (Mouse); 81760 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human GRK1 Leu343-Ser563 Accession # Q15835 |
| 生物活性 | Human, Mouse, Rat |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | G protein-coupled receptor kinases (GRKs) are important modulators of G protein-coupled receptor (GPCR) signaling. Receptor phosphorylation by specific GRKs plays a key role in triggering rapid desensitization. The GRK family consists of 7 isoforms that share a central catalytic domain with homology to other serine/threonine kinases. The catalytic domain is flanked by an amino-terminal RGS domain of 183‑188 amino acids and a carboxyl-terminus of variable length. GRK1 and GRK7 are members of the rhodopsin kinase or visual GRK subfamily. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Human/Mouse/Rat GRK1 by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, Ramos human Burkitts lymphoma cell line, PT18 mouse mast/basophil cell line, and L6 rat myoblast cell line. PVDF membrane was probed with 1 µg/mL of Human/Mouse/Rat GRK1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4498) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for GRK1 at approximately 63 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 1. |
