Human Apolipoprotein C-II Affinity Purified Polyclonal Ab (25 UG)【科瑞奥博】

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Human Apolipoprotein C-II Affinity Purified Polyclonal Ab (25 UG)

货号： AF4497-SP 基本售价： 1378.1 元规格： -

产品信息

概述
货号	AF4497-SP
别名	APC2; APOC2; Apo-CII; apoC-II; Apolipoprotein C2; apolipoprotein C-II; MGC75082
反应种属	Human
应用	Western Blot(0.5 µg/mL) Simple Western(5 µg/mL)
目标/特异性	Detects human ApoC2 in Western blots. In Western blots, this antibody detects both the precursor and processed forms of ApoC2.
使用方法	Western Blot: 0.5 µg/mL Simple Western: 5 µg/mL
来源	Reconstitute at 0.2 mg/mL in sterile PBS.
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	344 (Human); 11813 (Mouse); 292697 (Rat)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Amphipathic alpha-helices in apolipoproteins are crucial to the formation of infectious hepatitis C virus particles. Authors: Fukuhara T, Wada M, Nakamura S, Ono C, Shiokawa M, Yamamoto S, Motomura T, Okamoto T, Okuzaki D, Yamamoto M, Saito I, Wakita T, Koike K, Matsuura Y PLoS Pathog, 2014;10(12):e1004534. Species: Human Sample Type: Cell Lysate Application: WB
纯化方式	Antigen Affinity-purified
免疫原	E. coli-derived recombinant human ApoC2 Thr23-Glu101 Accession # P02655
生物活性	Human
标记	Unconjugated
溶解方法	Reconstitute at 0.2 mg/mL in sterile PBS.
背景	Apolipoprotein C2 (ApoC2/II) is a 8‑9 kDa, secreted member of the Apolipoprotein C2 family of proteins. It is produced by hepatocytes and represents a major component of VLDL particles. It activates lipoprotein lipase and may self-associate to form amyloid-type fibrils. The human ApoC2 precursor is 101 amino acids (aa) in length. It contains a 22 amino acid (aa) signal sequence, followed by a 79 aa ProApoC2 that contains a lipid-binding region (aa 43‑51) and an enzyme interaction site (aa 55‑78). ProApoC2 represents >90% of circulating ApoC2. In human, limited proteolytic processing occurs with removal of the six aa prosegment (aa 23‑28). This does not affect activity. Human ProApoC2 is 59% and 62% aa identical to mouse and rat ProApoC2, respectively.
运输条件	Blue Ice
存放说明	4℃
参考文献	ApoE Suppresses Cyclophilin A to Maintain Cerebrovascular Integrity: A Pharmacological Target for Alzheimer’s Disease Lipid & Fatty Acid Transport Lipid/Cholesterol Metabolism Obesity

参考图片

Detection of Human Apolipoprotein C-II/ApoC2 by Western Blot. Western blot shows lysates of human small intestine tissue and plasma. PVDF membrane was probed with 0.5 µg/mL of Human Apolipoprotein C-II/ApoC2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4497) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for precursor Apolipoprotein C-II/ApoC2 at approximately 12 kDa and processed ApoC2 at approximately 8 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

Detection of Human
Apolipoprotein C‑II/ApoC2 by Simple Western^TM. Simple Western lane view shows lysates of human plasma, loaded at 0.2 mg/mL. A specific band was detected for Apolipoprotein C‑II/ApoC2 at approximately 9 kDa (as indicated) using 5 µg/mL of Sheep Anti-Human Apolipoprotein C‑II/ApoC2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4497) followed by 1:50 dilution of HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). This experiment was conducted under reducing conditions and using the
12-230 kDa separation system.