| 货号 | AF4496-SP |
| 别名 | ABCDS; endothelin B receptor; Endothelin receptor non-selective type; endothelin receptor type B; ETB; ET-B; ETBR; ET-BR; ETRB; HSCR; HSCR2; WS4A | 全称 | Endothelin Receptor Type B |
| 反应种属 | Human |
| 应用 | Western Blot(1 µg/mL) Flow Cytometry(2.5 µg/106cells) Immunocytochemistry(5-15 µg/mL) |
| 目标/特异性 | Detects human EDNRB in direct ELISAs and Western blots. |
| 使用方法 | Western Blot: 1 µg/mL Flow Cytometry: 2.5 µg/106cells Immunocytochemistry: 5-15 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 1910 (Human); 13618 (Mouse); 50672 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human EDNRB Glu27-Lys101 Accession # P24530 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | EDNRB (Endothelin B Receptor) is a member of the beta-family of rhodopsin receptors. It binds endothelin 1, 2, and 3 and is found on endothelial cells where it mediates vasodilation. Mature human EDNRB is a 7-transmembrane glycoprotein that is 416 amino acids (aa) in length. It contains a 75 aa N-terminal extracellular region (aa 27-101), and a 44 aa C-terminal cytoplasmic domain. There are three EDNRB variants that affect aa 27-101. One shows a 90 aa N-terminal extension, a second shows the same 90 aa N-terminal substitution coupled with a deletion of aa 268-398, and a third shows proteolytic cleavage between Arg64-Ser65. Over aa 27-101, human EDNRB shares 67% and 97% aa sequence identity with mouse and canine EDNRB, respectively. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Human EDNRB/Endothelin R Type B by Western Blot. Western blot shows lysates of HEK293 human embryonic kidney cell line, A172 human glioblastoma cell line, and Raji human Burkitts lymphoma cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human EDNRB/Endothelin R Type B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4496) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for EDNRB/Endothelin R Type B at approximately 38 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8. | |
Detection of EDNRB/Endothelin R Type B in A172 Human Cell Line by Flow Cytometry. A172 human glioblastoma cell line was stained with Human EDNRB/Endothelin R Type B Antigen Affinity‑purified Polyclonal Antibody (Catalog # AF4496, filled histogram) or isotype control antibody (Catalog # 5‑001‑A, open histogram), followed by NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # NL010). | |
EDNRB/Endothelin R Type B in A172 Human Cell Line. EDNRB/Endothelin R Type B was detected in immersion fixed A172 human glioblastoma cell line using 10 µg/mL Sheep Anti-Human EDNRB/Endothelin R Type B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4496) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips. |
