| 货号 | AF4466-SP |
| 别名 | EC 3.1.4.11; EIEE12; FLJ45792; inositoltrisphosphohydrolase; KIAA05811-phosphatidylinositol-4,5-bisphosphate phosphodiesterase beta 1; monophosphatidylinositol phosphodiesterase; phosphoinositidase C; Phosphoinositide phospholipase C-beta-1; phospholipase C, beta 1 (phosphoinositide-specific); Phospholipase C-beta-1; Phospholipase C-I; PI-PLC; PLC-154; PLC154,1-phosphatidylinositol-4,5-bisphosphate phosphodiesterase beta-1; PLCB1; PLCB1A; PLCB1B; PLC-beta-1; PLC-I; PLC-I1-phosphatidyl-D-myo-inositol-4,5-bisphosphate; triphosphoinositide phosphodiesterase | 全称 | Phospholipase C beta 1 |
| 反应种属 | Human/Mouse |
| 应用 | Western Blot(1 µg/mL) |
| 目标/特异性 | Detects human and mouse PLC-beta 1, but does not detect recombinant human (rh) PLC-beta 2, rhPLC-beta 3, or rhPLC-beta 4 in Western blots. |
| 使用方法 | Western Blot: 1 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 23236 (Human); 18795 (Mouse); 24654 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human PLC-beta 1 Lys27-Met245 Accession # Q9NQ66 |
| 生物活性 | Human, Mouse |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | The Phospholipase C family consists of 13 isozymes within six subfamilies, PLC-δ, -beta, -gamma, -epsilon, -zeta, and -eta. PLC-beta 1 (Phospholipase C beta-1) is a G-protein dependent phosphodiesterase that catalyzes the generation of inositol 1, 4, 5-trisphosphate (IP3) and diacylglycerol (DAG) from phosphatidylinositol 4, 5-bisphosphate (IP2), an essential step in the intracellular transduction of many extracellular signals. Alternative splicing results in two transcript variants encoding two isoforms, PLC-beta 1A and PLC-beta 1B. PLC-beta 1A is preferentially expressed in the cytosol, while PLC-beta 1B is predominantly expressed in the nucleus. The region used as immunogen is 100% identical in both isoforms. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Human/Mouse PLC‑ beta 1 by Western Blot. Western blot shows lysates of human brain tissue and mouse brain tissue. PVDF membrane was probed with 1 µg/mL Sheep Anti-Human/Mouse PLC‑ beta 1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4466) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band for PLC‑ beta 1 was detected at approximately 150 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. | |
Detection of Human PLC‑ beta 1 by Western Blot. Western blot shows recombinant human PLC-beta 1, PLC-beta 2, PLC-beta 3, PLC-beta 4 (5 ng/lane). PVDF membrane was probed with 1 µg/mL Sheep Anti-Human/Mouse PLC‑ beta 1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4466) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band for PLC‑ beta 1 was detected at approximately 28 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. |
