| 货号 | AF4455-SP |
| 别名 | activating transcription factor 2formerly; cAMP responsive element binding protein 2; cAMP-responsive element-binding protein 2; CREB2; CREBP1; CRE-BP1; cyclic AMP-dependent transcription factor ATF-2; HB16; MGC111558; mXBP; TREB7 | 全称 | Activating Transcription Factor 2 |
| 反应种属 | Human/Mouse |
| 应用 | Western Blot(1 µg/mL) |
| 目标/特异性 | Detects human and mouse ATF2 in Western blots. |
| 使用方法 | Western Blot: 1 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 1386 (Human); 11909 (Mouse); 81647 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human ATF2 Val270-Ser505 Accession # P15336 |
| 生物活性 | Human, Mouse |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | ATF2 is a member of the bzip family of transcription factors. ATF2 responds to cAMP activation in cells by either homo or hetero dimerizing with c-Jun and binding to cAMP response elements (TGACGTCA). ATF2 also contains histone acetylase activity that can be induced by ionizing radiation. ATF2 can be phosphorylated by a variety of kinases (JNK, p38, ATM), and this phosphorylation correlates with increased activity. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Human/Mouse ATF2 by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, MCF‑7 human breast cancer cell line, and NIH‑3T3 mouse embryonic fibroblast cell line. PVDF membrane was probed with 1 µg/mL of Human/Mouse ATF2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4455) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for ATF2 at approximately 80 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3. |
