| 货号 | AF4435-SP |
| 别名 | BD14; BD3; BD-3; beta-defensin 103; Beta-defensin 3; DEFB103; DEFB103A; DEFB103B; DEFB3; DEFB-3; DEFB3DEFB103A; Defensin, beta 103; defensin, beta 103B; Defensin-like protein; HBD-3; HBD3HBP3 |
| 反应种属 | Human |
| 应用 | Western Blot(0.1 µg/mL) |
| 目标/特异性 | Detects human beta -Defensin 3 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 1% cross-reactivity with recombinant human beta -Defensin 2 is observed. |
| 使用方法 | Western Blot: 0.1 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 55894 (Human) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human beta -Defensin 3 Gly23-Lys67 Accession # P81534 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | beta -Defensin 3, also known as BD3 and DEFB-3, is a membrane-active cationic peptide that functions in inflammation and innate immune responses. There are at least 30 beta ‑Defensins which are distinguished from alpha ‑Defensins by the connectivity pattern of their three intramolecular disulfide bonds (1). The 45 amino acid (aa) mature human BD3 shares 38% and 33% aa sequence identity with mouse and rat BD3, respectively (2, 3). It shares 18%‑36% aa sequence identity with other human beta ‑Defensins. BD3 is widely expressed among epithelial tissues, notably by keratinocytes and airway epithelial cells. It is up‑regulated in response to proinflammatory cytokines, microbial and viral infections, and at the edges of skin wounds (2, 4‑6). BD3 induction in osteoarthritis chondrocytes promotes MMP1 and 13 production and inhibits TIMP1 and 2 expression (7). In vivo control of BD3 activity is accomplished in part through cleavage by cathepsins B, L, and S (8). BD3 displays strain specific microbicidal activity toward a broad spectrum of bacteria and yeast (2, 9). BD3 also induces monocyte migration, mast cell activation, and a mast cell‑dependent increase in vascular permeability (4, 10). Disruption of the intramolecular disulfide bond pattern in BD3 abrogates its monocyte chemoattractant properties but not its antimicrobial properties (11, 12). BD3 inhibits viral infectivity by interacting directly with HIV-1 plus its coreceptor CXCR4 (5, 13), and with HSV glycoprotein B plus its receptor heparan sulfate (14), and by forming a protective coating on the surface of influenza virus target cells (15). |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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