货号 | AF4415-SP |
别名 | ARSB; arylsulfatase B; ASB; EC 3.1.6.12; G4S; MPS6; N-acetylgalactosamine-4-sulfatase |
反应种属 | Human |
应用 | Western Blot(1 µg/mL) Simple Western(10 µg/mL) Immunohistochemistry(5-15 µg/mL) |
目标/特异性 | Detects human Arylsulfatase B/ARSB in Western blots. |
使用方法 | Western Blot: 1 µg/mL Simple Western: 10 µg/mL Immunohistochemistry: 5-15 µg/mL |
来源 | Sterile PBS to a final concentration of 0.2 mg/mL. |
产品组分 |
供应商 | R&D Systems |
Entrez Gene IDs | 411 (Human); 11881 (Mouse); 25227 (Rat) |
纯化方式 | Antigen Affinity-purified |
免疫原 | Chinese hamster ovary cell line CHO-derived recombinant human Arylsulfatase B/ARSB Ser37-Met533 (Val376Met) Accession # P15848 |
生物活性 | Human |
标记 | Unconjugated |
溶解方法 | Sterile PBS to a final concentration of 0.2 mg/mL. |
背景 | ARSB, also known as N-acetylgalactosamine-4-sulfatase, is a lysosomal glycoprotein and one of at least nine human arylsulfatases that catalyze the hydrolysis of sulfate ester bonds. ARSB deficiency is causative of the lysosomal storage disease Mucopolysaccharidosis VI. Human ARSB is cleaved intracellularly into fragments which remain associated with a disulfide linkage. Mature human ARSB (aa 37-533) shares 80% amino acid sequence identity with mouse and rat ARSB. |
运输条件 | Blue Ice |
存放说明 | 4℃ |
参考文献 |
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Detection of Human Arylsulfatase B/ARSB by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line, Huh‑7 human hepatoma cell line, and human skeletal muscle tissue. PVDF Membrane was probed with 1 µg/mL of Sheep Anti-Human Arylsulfatase B/ARSB Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4415) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Arylsulfatase B/ARSB at approximately 52 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. | |
Arylsulfatase B/ARSB in Human Liver. Arylsulfatase B/ARSB was detected in immersion fixed paraffin-embedded sections of human liver using Sheep Anti-Human Arylsulfatase B/ARSB Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4415) at 3 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to endothelial cells in bile canaliculi. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections. | |
Detection of Human Arylsulfatase B/ARSB by Simple WesternTM. Simple Western lane view shows lysates of HepG2 human hepatocellular carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for Arylsulfatase B/ARSB at approximately 59 kDa (as indicated) using 10 µg/mL of Sheep Anti-Human Arylsulfatase B/ARSB Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4415) followed by 1:50 dilution of HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). This experiment was conducted under reducing conditions and using the12-230 kDa separation system. |